Transfer RNA translocation through the ribosome

Blau, Christian

Combining large scale systems simulations with experimental data

dc.contributor.advisor	Grubmüller, Helmut Prof. Dr.
dc.contributor.author	Blau, Christian
dc.date.accessioned	2015-02-24T10:20:33Z
dc.date.available	2015-02-24T10:20:33Z
dc.date.issued	2015-02-24
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0022-5DCE-9
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4906
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4906
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4906
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	530	de
dc.title	Transfer RNA translocation through the ribosome	de
dc.title.alternative	Combining large scale systems simulations with experimental data	de
dc.type	doctoralThesis	de
dc.contributor.referee	Grubmüller, Helmut Prof. Dr.
dc.date.examination	2014-03-05
dc.subject.gok	Physik (PPN621336750)	de
dc.description.abstracteng	During the elongation cycle, ribosomes create peptides from an mRNA blueprint. To prime the ribosome for a new round of elongation after peptide bond formation, the transfer RNA (tRNA) bound to the aminoacyl site (A site) moves to the peptidyl site (P site) while the tRNA bound to the P site moves to the exit site (E site). In this work we investigated the driving forces and dynamics of this process called tRNA translocation. We found surprisingly large kinetic rates for 30S body and head as well as L1-stalk movements during translocation, while the tRNA are "road-blocks" for most individual transitions. Further, we find that tRNA movement is aided by three distinct interaction modes with ribosomal proteins L1, L5 and L16: pulling, sliding and stepping. In the context of this analysis we developed an analysis tool to efficiently find conacting atoms in biomolecular ensemble data.	de
dc.contributor.coReferee	Stark, Holger Prof. Dr.
dc.contributor.thirdReferee	Enderlein, Jörg Prof. Dr.
dc.subject.eng	ribosome	de
dc.subject.eng	cryo-EM	de
dc.subject.eng	molecular dynamics	de
dc.subject.eng	refinement	de
dc.subject.eng	translocation	de
dc.subject.eng	tRNA	de
dc.subject.eng	range search	de
dc.subject.eng	rate estimates	de
dc.subject.eng	rate theory	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5DCE-9-3
dc.affiliation.institute	Fakultät für Physik	de
dc.identifier.ppn	818820098

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