• Deutsch
    • English
  • English 
    • Deutsch
    • English
  • Login
Item View 
  •   Home
  • Medizin
  • Human- und Zahnmedizin
  • Item View
  •   Home
  • Medizin
  • Human- und Zahnmedizin
  • Item View
JavaScript is disabled for your browser. Some features of this site may not work without it.

In-vivo -Monitoring der Effekte von Östrogen, Daidzein und 4-MBC mittels Flächendetektor-Volumen-CT am Modell der ovarektomierten Ratte

In vivo monitoring of the effects of estrogen, daidzein, and 4-MBC using Flat Panel Volumetric Computed Tomography on the ovariectomized rat

by Thomas Grüger
Doctoral thesis
Date of Examination:2014-01-14
Date of issue:2014-01-14
Advisor:PD Dr. Stephan Sehmisch
Referee:Prof. Dr. Hubertus Jarry
Referee:Prof. Dr. Martin Oppermann
crossref-logoPersistent Address: http://dx.doi.org/10.53846/goediss-4314

 

 

Files in this item

Name:PromoGrueger 140114.pdf
Size:1.51Mb
Format:PDF
ViewOpen

The following license files are associated with this item:


Abstract

English

The study investigated the influence of 4-methylbenzylidene camphor (4-MBC), daidzein, and estradiol-17β-benzoate (E(2)) on either intact or osteotomized cancellous bone in ovariectomized (Ovx) rats. Three-month old Ovx rats were fed with soy-free (SF) diet over 8 weeks; thereafter, bilateral transverse metaphyseal osteotomy of tibia was performed and rats were divided into groups: rats fed with SF diet and SF diet supplemented with 4-MBC (200 mg), daidzein (50 mg), or E(2) (0.4 mg) per kilogram body weight. After 5 or 10 weeks, computed tomographical, biomechanical, histological, and ashing analyses were performed in lumbar spine and tibia of 12 rats from each group. 4-MBC and E(2) improved bone parameters in lumbar spine and tibia, were not favorable for osteotomy healing, and decreased serum osteocalcin level. However, daidzein improved bone parameters to a lesser extent and facilitated osteotomy healing. For lumbar spine, the bone mineral density was 338±9, 346±5, 361±6, and 360±5 mg/cm(3) in SF, daidzein, 4-MBC, and E(2), respectively, after 10 weeks. For tibia, the yield load was 98±5, 114±3, 90±2, and 52±4 N in SF, daidzein, 4-MBC, and E(2), respectively, after 10 weeks. Serum daidzein was 54±6 ng/ml in daidzein group and equol was not detected. Alp and Igf1 genes were down-regulated in callus after daidzein and E(2) compared with 4-MBC (week 5). The response of bone tissue and serum markers of bone metabolism could be ordered: daidzein<4-MBC
Keywords: osteoporosis; 4-MBC; Daidzein; Flat Panel Volumetric Computed Tomography (fpVCT); ovariectomized rat
Schlagwörter: Osteoporose; 4-MBC; Daidzein; Flächendetektor-Volumen-Computertomographie (fpVCT)
 

Statistik

Publish here

Browse

All of eDissFaculties & ProgramsIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesTypeThis FacultyIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesType

Help & Info

Publishing on eDissPDF GuideTerms of ContractFAQ

Contact Us | Impressum | Data Protection Information
Göttingen State and University Library | Göttingen University