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In-vivo -Monitoring der Effekte von Östrogen, Daidzein und 4-MBC mittels Flächendetektor-Volumen-CT am Modell der ovarektomierten Ratte

In vivo monitoring of the effects of estrogen, daidzein, and 4-MBC using Flat Panel Volumetric Computed Tomography on the ovariectomized rat

Thomas Grüger
Doctoral thesis
Date of Examination: 2014-01-14
Date of issue: 2014-01-14
Advisor: Sehmisch, Stephan PD Dr.
Referee: Jarry, Hubertus Prof. Dr.
Referee: Oppermann, Martin Prof. Dr.

Persistent Address: http://hdl.handle.net/11858/00-1735-0000-0022-5DF2-6

 

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Abstract

English

The study investigated the influence of 4-methylbenzylidene camphor (4-MBC), daidzein, and estradiol-17β-benzoate (E(2)) on either intact or osteotomized cancellous bone in ovariectomized (Ovx) rats. Three-month old Ovx rats were fed with soy-free (SF) diet over 8 weeks; thereafter, bilateral transverse metaphyseal osteotomy of tibia was performed and rats were divided into groups: rats fed with SF diet and SF diet supplemented with 4-MBC (200 mg), daidzein (50 mg), or E(2) (0.4 mg) per kilogram body weight. After 5 or 10 weeks, computed tomographical, biomechanical, histological, and ashing analyses were performed in lumbar spine and tibia of 12 rats from each group. 4-MBC and E(2) improved bone parameters in lumbar spine and tibia, were not favorable for osteotomy healing, and decreased serum osteocalcin level. However, daidzein improved bone parameters to a lesser extent and facilitated osteotomy healing. For lumbar spine, the bone mineral density was 338±9, 346±5, 361±6, and 360±5 mg/cm(3) in SF, daidzein, 4-MBC, and E(2), respectively, after 10 weeks. For tibia, the yield load was 98±5, 114±3, 90±2, and 52±4 N in SF, daidzein, 4-MBC, and E(2), respectively, after 10 weeks. Serum daidzein was 54±6 ng/ml in daidzein group and equol was not detected. Alp and Igf1 genes were down-regulated in callus after daidzein and E(2) compared with 4-MBC (week 5). The response of bone tissue and serum markers of bone metabolism could be ordered: daidzein<4-MBC
Keywords: osteoporosis; 4-MBC; Daidzein; Flat Panel Volumetric Computed Tomography (fpVCT); ovariectomized rat
Schlagwörter: Osteoporose; 4-MBC; Daidzein; Flächendetektor-Volumen-Computertomographie (fpVCT)
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