In vivo Optical Imaging zum Nachweis der Leberrepopulation nach Konditionierung der Empfängerleber und Hepatozytentransplantation

Seif Amir Hosseini, Ali

dc.contributor.advisor	König, Sarah PD Dr.
dc.contributor.author	Seif Amir Hosseini, Ali
dc.date.accessioned	2014-05-23T07:53:31Z
dc.date.available	2014-06-28T22:50:05Z
dc.date.issued	2014-05-23
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0022-5EC4-6
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4514
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	In vivo Optical Imaging zum Nachweis der Leberrepopulation nach Konditionierung der Empfängerleber und Hepatozytentransplantation	de
dc.type	doctoralThesis	de
dc.title.translated	Noninvasive Imaging of Liver Repopulation Following Hepatocyte Transplantation	de
dc.contributor.referee	Alves, Frauke Prof. Dr.
dc.date.examination	2014-06-18
dc.description.abstracteng	Near infrared fluorescence (NIRF) optical imaging is a technique particularly powerful when studying in vivo processes at the molecular level in preclinical animal models. The purpose of this study was to assess optical imaging and the feasibility of donor cell expansion tracking in vivo using a fluorescent probe. Livers of dipeptidylpeptidase IV (DPPIV)-deficient rats were preconditioned with irradiation. Four days later, a partial hepatectomy was performed and wild-type (DPPIV+) hepatocytes were transplanted into recipient livers via the spleen. Repopulation by transplanted DPPIV+ hepatocytes was detected in vivo with Cy5.5-conjugated DPPIV antibody using the Optix MX2 System (ART, Montreal, Canada). Results were compared with nontransplanted control animals and transplanted animals receiving nonspecific antibody. Optical imaging detected Cy5.5-specific fluorescence in the liver region of the transplanted animals, increasing in intensity with time, representing extensive host liver repopulation within 16 weeks following transplantation. A general pattern of donor cell multiplication emerged, with an initially accelerating growth curve and later plateau phase. In contrast, no specific fluorescence was detected in the control groups. Comparison with ex vivo immunofluorescence staining of liver sections confirmed the optical imaging results. Optical imaging constitutes a potent method of assessing the longitudinal kinetics of liver repopulation in the rat transplantation model. Our results provide a basis for the future development of clinical protocols for suitable fluorescent dyes and imaging technologies.	de
dc.contributor.coReferee	Schön, Margarete Prof. Dr.
dc.subject.eng	In vivo optical imaging	de
dc.subject.eng	Near infrared dye Cy5.5	de
dc.subject.eng	Irradiation	de
dc.subject.eng	Rat liver repopulation	de
dc.subject.eng	DPPIV	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0022-5EC4-6-6
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Radiologie / Bildgebende Verfahren / Ultraschall / Nuklearmedizin / Strahlenschutz - Allgemein- und Gesamtdarstellungen (PPN619875585)	de
dc.subject.gokfull	Chirurgie - Allgemein- und Gesamtdarstellungen (PPN619875968)	de
dc.description.embargoed	2014-06-28
dc.identifier.ppn	786416297

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Human- und Zahnmedizin [2873]

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