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In vivo Optical Imaging zum Nachweis der Leberrepopulation nach Konditionierung der Empfängerleber und Hepatozytentransplantation

dc.contributor.advisorKönig, Sarah PD Dr.
dc.contributor.authorSeif Amir Hosseini, Ali
dc.titleIn vivo Optical Imaging zum Nachweis der Leberrepopulation nach Konditionierung der Empfängerleber und Hepatozytentransplantationde
dc.title.translatedNoninvasive Imaging of Liver Repopulation Following Hepatocyte Transplantationde
dc.contributor.refereeAlves, Frauke Prof. Dr.
dc.description.abstractengNear infrared fluorescence (NIRF) optical imaging is a technique particularly powerful when studying in vivo processes at the molecular level in preclinical animal models. The purpose of this study was to assess optical imaging and the feasibility of donor cell expansion tracking in vivo using a fluorescent probe. Livers of dipeptidylpeptidase IV (DPPIV)-deficient rats were preconditioned with irradiation. Four days later, a partial hepatectomy was performed and wild-type (DPPIV+) hepatocytes were transplanted into recipient livers via the spleen. Repopulation by transplanted DPPIV+ hepatocytes was detected in vivo with Cy5.5-conjugated DPPIV antibody using the Optix MX2 System (ART, Montreal, Canada). Results were compared with nontransplanted control animals and transplanted animals receiving nonspecific antibody. Optical imaging detected Cy5.5-specific fluorescence in the liver region of the transplanted animals, increasing in intensity with time, representing extensive host liver repopulation within 16 weeks following transplantation. A general pattern of donor cell multiplication emerged, with an initially accelerating growth curve and later plateau phase. In contrast, no specific fluorescence was detected in the control groups. Comparison with ex vivo immunofluorescence staining of liver sections confirmed the optical imaging results. Optical imaging constitutes a potent method of assessing the longitudinal kinetics of liver repopulation in the rat transplantation model. Our results provide a basis for the future development of clinical protocols for suitable fluorescent dyes and imaging
dc.contributor.coRefereeSchön, Margarete Prof. Dr.
dc.subject.engIn vivo optical imagingde
dc.subject.engNear infrared dye Cy5.5de
dc.subject.engRat liver repopulationde
dc.affiliation.instituteMedizinische Fakultätde
dc.subject.gokfullRadiologie / Bildgebende Verfahren / Ultraschall / Nuklearmedizin / Strahlenschutz - Allgemein- und Gesamtdarstellungen (PPN619875585)de
dc.subject.gokfullChirurgie - Allgemein- und Gesamtdarstellungen (PPN619875968)de

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