Die Wirkung von 20-OH-Ecdyson auf Osteoporose und das Fett im Kniegelenk im Zusammenhang mit dem Metabolischen Syndrom.
The effects of 20-OH-Ecdysone on osteoporosis and fat in the knee joint in connection with the metabolic syndrome.
by Marie Sunder-Plassmann
Date of Examination:2014-06-18
Date of issue:2014-06-10
Advisor:PD Dr. Dana Seidlová-Wuttke
Referee:Prof. Dr. Dirk Raddatz
Referee:Prof. Dr. Margarete Schön
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EnglishPostmenopausal women have an increasing risk of suffering from osteoporosis (Bord et al. 2003) and to developing the ‘metabolic syndrome’ (Carr 2003; Lobo 2008). These are two conditions with serious sequelae that are rising in importance globally. In recent years there has been investigations that showed a correlation between these diseases and the interaction of fat and bone metabolism (Hwang and Choi 2010). In order to develop alternatives with less side affects than the common hormone replacement therapy for women in postmenopause, there is research interest in the field of phytotherapy. So far, studies have proven that the Ecdysteroid 20-hydroxy-Ecdysone (Ecd) seems to have multiple positive effects. For example in bioassay it improved the menopause typical “hot flushes” (Puri et al. 2012) and prevented the development of osteoporosis and metabolic syndrome (Seidlová-Wuttke et al. 2010a; Seidlová-Wuttke et al. 2010b). This dissertation was structured to compile the literature and provide new original research on the effects of Ecd as a potential therapy for post-menopausal women Besides the investigation of Ecd and the reaction this has on bone, this work also analyses the influence of Ecd on the adjoining adipose tissue, in order to assess the interaction of fat and bone metabolism. The established model of ovariectomized (ovx) rats for the postmenopause woman was used. There have been four groups, each with ten rats. Three groups were ovx. Every ovx group got either soy free food, soy free food + Ecd or soy free food + 17-ß-Estradiol (E2). After 4 weeks they were decapitated and the primary investigator performed the histomorphological analysis. Analytical parameters of the investigations were the due of fat, trabecular tissue and red bone marrow in the metaphyse, thickness of corticalis, the epiphysial cartilage and the due of fat in the joint space. One of our first findings was that the thickness of corticalis of the tibia was maintained in the Ecd group. After four weeks giving Ecd it was significantly thicker than the ovx-group. In addition we found a significant loss of trabecular tissue in the metaphysis, which is an important component of osteoporosis, in the ovx-group. The Ecd-group showed a significant higher amount of trabecular tissue than the ovx-group. The bonemarker osteocalzin and crossLaps showed a higher bone metabolism after giving Ecd. This encourages the hypothesis that the anti osteoporosic effect of Ecd is based on increasing bone structure, dislike E2 which enacts this through decreasing loss of bone structure (Kapur et al. 2010). Seemingly Ecd also has a protective effect concerning the increase of fat mass. The part of fat in the bone marrow of the metaphysis was significantly less than that in the ovx-group. Therefore the adjoining bone is less exposed to lipotoxication, which is a result of a higher production of cytokines of the adipozytes (Cao 2011). Also the due of fat in the joint space in the Ecd-group was significant lower. In consideration of the results of the department of endocrinology Göttingen, which showed that the joint cartilage was significantly wider in the Ecd- and the E2-groups, it can lead to the conclusion that Ecd and the metabolic effects of it on fat may also protect against osteoarthritis. We would recommend further research to confirm and add to the findings of our study. Ecd additionally confirmed it’s already known proliferative effects. The epiphysial cartilage in Ecd-group was significant wider and the due of red bone marrow in the metaphysis was significant higher than in the ovx-group for our sample of rats. Because of the interaction of fat- and bone metabolism osteoporosis could be named as a “metabolic syndrome of the bone”. This study reveals that Ecd has not just a positive effect on the bone, it also has a protective influence on the development of the adjoining adipose tissue. That means that Ecd may have the potential to influence various sources of the development of osteoporosis and may also be able to prevent it.
Keywords: osteoporosis; metabolic syndrome; ecdysone; postmenopause
Schlagwörter: Osteoporose; Metabolisches Syndrom; Ecdyson; Postmenopause