Scanning X-Ray Nanodiffraction on Dictyostelium discoideum

Priebe, Marius Patrick

dc.contributor.advisor	Salditt, Tim Prof. Dr.
dc.contributor.author	Priebe, Marius Patrick
dc.date.accessioned	2015-11-06T10:25:48Z
dc.date.available	2015-11-06T10:25:48Z
dc.date.issued	2015-11-06
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0023-967B-2
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-5183
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	530	de
dc.title	Scanning X-Ray Nanodiffraction on Dictyostelium discoideum	de
dc.type	doctoralThesis	de
dc.contributor.referee	Salditt, Tim Prof. Dr.
dc.date.examination	2015-02-04
dc.subject.gok	Physik (PPN621336750)	de
dc.description.abstracteng	In the recent years, novel focussing optics for synchrotron radiation sources became available, which now allow focussing the x-radiation down to 100\,nm (FWHM). Thus, the typically poor spatial resolution of Small Angle X-ray Scattering (SAXS) has been overcome and enables "Scanning X-ray Nanodiffraction", where a sample is scanned in a linewise motion, while recording a far-field diffraction pattern at every scanning position. In this work, Scanning Nanodiffraction was applied to single cells of the amoeba \textit{Dictyostelium discoideum}, which is a model system for amoeboid migration, that is enabled by a reorganization of cytoskeletal biopolymers in the cell cortex. The spatially resolved small angle x-ray diffraction signal shows characteristic streak-like patterns in reciprocal space, which is attributed to fibre bundles of the actomyosin network. An anisotropy parameter nicknamed "Streak Finder" is introduced to characterize the pronounced local variations within the cell. The x-ray differential phase contrast is evaluated in terms of the projected electron density and additional x-ray fluorescence aquisitions provide information on the spatially resolved distribution (2D) of elements within the cell. The x-ray results are correlated with optical microscopy (phase contrast and fluorescence microscopy of strains with labelled actin and myosin II) on live, fixed, and cryogenic cells.	de
dc.contributor.coReferee	Enderlein, Jörg Prof. Dr.
dc.contributor.thirdReferee	Bodenschatz, Eberhard Prof. Dr.
dc.contributor.thirdReferee	Köster, Sarah Prof. Dr.
dc.contributor.thirdReferee	Techert, Simone Prof. Dr.
dc.contributor.thirdReferee	Hub, Jochen S. Dr.
dc.subject.eng	Nanodiffraction	de
dc.subject.eng	Dictyostelium discoideum	de
dc.subject.eng	Actin	de
dc.subject.eng	Myosin-II	de
dc.subject.eng	Streak Finder	de
dc.subject.eng	SAXS	de
dc.subject.eng	STXM	de
dc.subject.eng	Fluorescence mapping	de
dc.subject.eng	Ptychography	de
dc.subject.eng	X-ray	de
dc.subject.eng	Vitrification	de
dc.subject.eng	Frozen-hydrated cells	de
dc.subject.eng	Sample preparation	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0023-967B-2-9
dc.affiliation.institute	Fakultät für Physik	de
dc.identifier.ppn	842309098

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