• Deutsch
    • English
  • English 
    • Deutsch
    • English
  • Login
Item View 
  •   Home
  • Medizin
  • Human- und Zahnmedizin
  • Item View
  •   Home
  • Medizin
  • Human- und Zahnmedizin
  • Item View
JavaScript is disabled for your browser. Some features of this site may not work without it.

Der ALK-Rezeptor in zellulären Modellen des humanen Neuroblastomkrebses: Aktivierung und Signalisierung

The ALK receptor in cellular models of human neuroblastoma cancer: activation and signaling

by Laura Jacqueline Aksak
Doctoral thesis
Date of Examination:2018-01-31
Date of issue:2018-01-08
Advisor:Prof. Dr. Freddy Wouters-Bunt
Referee:Prof. Dr. Dieter Kube
Referee:Prof. Dr. Margarete Schön
crossref-logoPersistent Address: http://dx.doi.org/10.53846/goediss-6661

 

 

Files in this item

Name:diss221217mitlesezeichenfüredissoptimiert.pdf
Size:13.8Mb
Format:PDF
ViewOpen

The following license files are associated with this item:


Abstract

English

This thesis is about different mutations of the tyrosine kinase ALK and their role in the human neuroblastoma cancer. The ALK subtypes wildtype, F1174L, R1275Q, Y1604F and I1250T have been investigated in a laboratory projekt using neuroblastoma cells, fluorescence markers and microscopy. On the one hand, different behaviour of the subtypes could be shown when it comes to activation and activation thresholds, measured by displaying phosphotyrosine as a marker of ALK activation, taking the amount of ALK receptors into account. This behaviour could be reflected in FRET measurements. In addition, the ALK-subtypes showed typical cytomorphology, that was partiallly first described and compared. On the other hand, the downstream signaling of the ALK subtypes was analyzed by using sandwich immuno assays, thereby prooving a leading role of the Akt pathway. The Ras/Raf/MEK/ERK pathway was found to play a minor role in the ALK downstream signaling, which was additionally confirmed using an ERK1/2 inhibitor on neuroblastoma cells, that showed no effect on the amount and distribution of phosphotyrosine, the marker of ALK activation.
Keywords: neuroblastoma; alk
Schlagwörter: Neuroblastom; ALK
 

Statistik

Publish here

Browse

All of eDissFaculties & ProgramsIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesTypeThis FacultyIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesType

Help & Info

Publishing on eDissPDF GuideTerms of ContractFAQ

Contact Us | Impressum | Cookie Consents | Data Protection Information | Accessibility
eDiss Office - SUB Göttingen (Central Library)
Platz der Göttinger Sieben 1
Mo - Fr 10:00 – 12:00 h


Tel.: +49 (0)551 39-27809 (general inquiries)
Tel.: +49 (0)551 39-28655 (open access/parallel publications)
ediss_AT_sub.uni-goettingen.de
[Please replace "_AT_" with the "@" sign when using our email adresses.]
Göttingen State and University Library | Göttingen University
Medicine Library (Doctoral candidates of medicine only)
Robert-Koch-Str. 40
Mon – Fri 8:00 – 24:00 h
Sat - Sun 8:00 – 22:00 h
Holidays 10:00 – 20:00 h
Tel.: +49 551 39-8395 (general inquiries)
Tel.: +49 (0)551 39-28655 (open access/parallel publications)
bbmed_AT_sub.uni-goettingen.de
[Please replace "_AT_" with the "@" sign when using our email adresses.]