Cell-cell communication between chondrocytes and osteoblasts through miR-221-3p loaded extracellular vesicles

Shang, Xiaobin

dc.contributor.advisor	Schilling, Arndt Prof. Dr
dc.contributor.author	Shang, Xiaobin
dc.date.accessioned	2022-08-30T07:58:10Z
dc.date.available	2022-09-22T00:50:13Z
dc.date.issued	2022-08-30
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/14226
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-9414
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc	610	de
dc.title	Cell-cell communication between chondrocytes and osteoblasts through miR-221-3p loaded extracellular vesicles	de
dc.type	doctoralThesis	de
dc.contributor.referee	Schilling, Arndt Prof. Dr
dc.date.examination	2022-09-15	de
dc.description.abstracteng	Osteoarthritis (OA) is a whole joint disease manifested by cartilage degeneration and subchondral bone remodeling, and bone-cartilage communication plays a vital role in the disease progression with the existence of microchannels across the bone-cartilage interface. However, the knowledge of how the signal transduction from chondrocytes to osteoblasts happens is limited. Recently, miR-221-3p was demonstrated to be mechanosensitive in cartilage chondrocytes and extracellular vesicles (EVs) have been deeply researched for a specific role in cell-cell communication. In the present study, we investigated the role of EVs in chondrocyte-osteoblast communication by transferring the signal of miR-221-3p. EVs were isolated from the supernatant of chondrocytes by the PEG precipitation method and identified with nanoparticle tracking analysis (NTA) and western blot. The results suggest that the chondrocytes secreted EVs not only mediate the communication of these two cells in a coculture model but also inhibit the bone formation capacity of osteoblasts via the cargo of miR-221-3p. Therefore, this study provides a novel perspective on the communication between chondrocytes and osteoblasts through EVs and this can be considered a reliable approach to modulate the bone-cartilage remodeling in the future.	de
dc.contributor.coReferee	Brockmeyer, Phillipp PD Dr.
dc.subject.ger	Osteoarthritis	de
dc.subject.ger	Extracellular vesicles	de
dc.subject.ger	miRNA	de
dc.subject.eng	Osteoarthritis	de
dc.subject.eng	Extracellular vesicles	de
dc.subject.eng	miRNA	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-14226-2
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Orthopädie (PPN619876204)	de
dc.description.embargoed	2022-09-22	de
dc.identifier.ppn	1815552867
dc.notes.confirmationsent	Confirmation sent 2022-08-30T08:15:02	de

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Human- und Zahnmedizin [2877]

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