Auswirkungen der Rezeptor-Dimerisierung auf das Calcium-imaging muskarinerger Acetylcholinrezeptoren M1, M3 und M5

Zengin, Ilya Edip

dc.contributor.advisor	Niebert, Marcus PD Dr.
dc.contributor.author	Zengin, Ilya Edip
dc.date.accessioned	2022-12-15T10:00:05Z
dc.date.available	2022-12-22T00:50:10Z
dc.date.issued	2022-12-15
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/14417
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-9614
dc.format.extent	91	de
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc	610	de
dc.title	Auswirkungen der Rezeptor-Dimerisierung auf das Calcium-imaging muskarinerger Acetylcholinrezeptoren M1, M3 und M5	de
dc.type	doctoralThesis	de
dc.contributor.referee	Oppermann, Martin Prof. Dr.
dc.date.examination	2022-12-15	de
dc.description.abstracteng	This dissertation focuses on the effects of receptor-dimerization regarding the muscarinic Acetylcholine-receptors M1, M3 and M5. The goal was to evaluate whether the dimerization between homo- or heterologous receptor partners between M1, M3, and M5 have an impact on the following intracellular receptor-signalling. We developed a method to enforce dimerization between two recepor-partners by generating receptors which have attached either an antigen (GFP) or an anti-GFP-antibody. By co-expressing both modified receptors on the cell surface we could ensure a forced dimerization between the two receptor partners due to direct antibody-antigen binding. We interpreted the impact of dimerization by measuring and comparing the intracellular calcium-levels in with live cell calcium-imaging. The results showed, that dimerization between two mAChRs can lead to potential coupling mechanism between the receptors and have an impact on the signalling of those.	de
dc.contributor.coReferee	Meyer, Thomas Prof. Dr.
dc.subject.eng	Dimerization	de
dc.subject.eng	mAChR	de
dc.subject.eng	Calcium-imaging	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-14417-3
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Physiologie / Pathophysiologie - Allgemein- und Gesamtdarstellungen (PPN619875283)	de
dc.description.embargoed	2022-12-22	de
dc.identifier.ppn	1827567082
dc.notes.confirmationsent	Confirmation sent 2022-12-15T10:15:01	de

Dateien

Name:Dissertation Ilya Zengin finale Version.pdf

Größe:5.826Mb

Format:PDF

Öffnen

Name:: Dissertation Ilya Zengin finale ...
Größe:: 5.826Mb
Format:: PDF

Öffnen

Das Dokument erscheint in:

Human- und Zahnmedizin [2873]

Zur Kurzanzeige