Verbesserung der MALDI-TOF-basierten Identifizierung humanpathogener Pilze durch die Erstellung individueller Datenbanken
von Mareike Yinka Bernhard
Datum der mündl. Prüfung:2023-12-12
Erschienen:2023-12-12
Betreuer:Prof. Dr. Uwe Groß
Gutachter:Prof. Dr. Uwe Groß
Gutachter:Prof. Dr. Simone Scheithauer
Dateien
Name:Bernhard, Mareike-Dissertation, Version für ...pdf
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Zusammenfassung
Englisch
The identification of rare fungal species using MALDI-TOF mass spectrometry is limited by the fact that commercial databases are incomplete or the preparation protocols in routine diagnostics are complex. This dissertation, which consists of three publications, shows that it is possible to use simple means to create your own databases that can close these gaps or can simplify work processes. In the first publication “Yeast On-Target Lysis (YOTL), a Procedure for Making Auxiliary Mass Spectrum Data Sets for Clinical Routine Identification of Yeasts” we show how a simplified sample processing protocol using a minimal database of 6 reference spectra simplifies the work in routine diagnostics could become. The second publication “Towards proteomic species barcoding of fungi - an example using Scedosporium / Pseudallescheria genus isolates” describes the creation of a database with isolates from the Scedosporia / Pseudallescheria complex, which can also be used in clinical routine. In the third publication “CryptoType – datasets for MALDI-TOF based differentiation of Cryptococcus neoformans/gattii complex isolates” we created a dataset of 13 reference spectra for the Biotyper MALDI-TOF MS, which consists of the seven recognized non-hybrid subtypes in the Cryptococcus neoformans / gattii complexes. This subtyping was previously not possible with commercial databases, but here we were able to show that self-created databases can close gaps in commercial databases. These three publications are intended to show the potential for improving the identification of rare fungal species by creating your own databases tailored to your own needs.
Keywords: MALDI-TOF; Candida species identification