Browsing Fakultät für Biologie und Psychologie (inkl. GAUSS) by Referee "Cramer, Patrick Prof. Dr."
Now showing items 1-10 of 10
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Multi-omics studies of RNA polymerase II transcription regulation by cyclin-dependent kinases
(2024-10-30)Phosphorylation of the C-terminal repeat domain (CTD) of RNA polymerase II (Pol II) plays a central role in the regulation of gene transcription in eukaryotes. In yeast and mammals, the CTD is highly conserved and consists ... -
Computational analysis of transcriptional regulation and chromatin assembly
(2023-12-07)Primordial germ cells (PGCs), the precursors of gametes, are specified from post-implantation epiblast cells in response to bone morphogenetic protein (BMP) signaling at the onset of gastrulation in mouse. Followed by ... -
Investigating chemical crosslinking of protein-RNA complexes by mass spectrometry
(2022-08-12)RNA plays a quintessential role in cellular processes such as gene expression, transcription, RNA processing, and translation. Proteins carrying out those diverse functions are inherently flexible to accommodate dynamic ... -
Biochemical characterization of human shieldin complex
(2022-08-01)Shieldin is a newly identified DNA repair effector involved in repair of double strand breaks (DSBs) in G1 phase of cell cycle. Shieldin is a four-component complex consisting of proteins SHLD1, SHLD2, SHLD3 and HORMA REV7. ... -
Bioinformatic analysis of multi-omics data elucidates transcriptional cyclin dependent kinase mediated transcriptional regulation
(2022-08-01)Transcriptional regulation is a highly dynamic biological process which is governed by a complex network of proteins for precise expression of a gene. One of the most important regulators of gene expression are cyclin ... -
Structural studies of short nucleosome arrays containing linker histone H1
(2022-04-21)The DNA inside the eukaryotic nucleus is bound by octamers of histone proteins to form the nucleosome as the basic repeating unit of chromatin. Along the genome, nucleosomes locally form regularly spaced arrays. The relative ... -
Reconstitution of the Integrator complex and its interaction with paused transcription elongation complex of Pol II-DSIF-NELF
(2021-10-19)The Integrator complex (INT) is the latest and largest multi-subunit protein complex to be added to the list of factors involved in RNA Polymerase II (Pol II) transcription. INT consist of 15 subunits with an estimated ... -
Structure of SWI/SNF chromatin remodeller RSC bound to a nucleosome and implications for chromatin remodelling
(2020-07-15)Eukaryotic cells condense their DNA into chromatin, which hampers the accessibility of genetic information. Nucleosomes are the smallest building blocks of chromatin and are comprised of an octameric core of histone proteins, ... -
Transcriptome maps of general eukaryotic RNA degradation factors and identification and functional characterization of the novel mRNA modification N<sup>3</sup>-methylcytidine
(2019-07-01)The first part of this dissertation focused on the different RNA degradation pathways in the yeast Saccharomyces cerevisiae (S. cerevisiae). We used the method photoactivatable ribonucleoside-enhanced crosslinking and ... -
Structure of the Pol II initation complex with TFIIH and core Mediator and mechanistic implications for transcription
(2018-03-13)The transcription of protein coding genes is a key step in the conversion of genomic information into the content of a cell’s proteome. In eukaryotes, this process is performed by the multi-subunit DNA-dependent RNA ...