| dc.contributor.advisor | Seitz, Cornelia Sabine Prof. Dr. | |
| dc.contributor.author | Priebe, Marie Kristin | |
| dc.date.accessioned | 2020-01-29T10:19:54Z | |
| dc.date.available | 2020-03-10T23:50:03Z | |
| dc.date.issued | 2020-01-29 | |
| dc.identifier.uri | http://hdl.handle.net/21.11130/00-1735-0000-0005-12FE-9 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-7813 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-7813 | |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject.ddc | 610 | de |
| dc.title | Differential responses of human melanoma cells to c-Rel downregulation | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Dobbelstein, Matthias Prof. Dr. | |
| dc.date.examination | 2020-03-03 | |
| dc.description.abstracteng | The transcription factor NF-κB, which is one of the central regulators regarding
cell growth, proliferation, apoptosis, inflammation and invasion, was shown to
also play a role in melanoma development and progression. This thesis aimed to
further enlighten the role of the NF-κB subunit c-Rel in malignant melanoma.
For this purpose, seven melanoma cell lines derived from primary tumor and
metastasis were characterized regarding c-Rel expression and growth pattern,
revealing varying c-Rel expression levels and growth features. Transient downregulation of c-Rel by siRNA in melanoma cell lines A375 (derived from primary
tumor) and LOX (derived from metastasis) led to growth inhibition, suggesting a
pro-proliferative function. Altered cell cycle distribution, namely increased
portions of cells in G2/M phase and increased numbers of polyploid cells were
detected after c-Rel down-regulation in both cell lines. Additionally, c-Rel downregulation leads to an altered mitotic spindle morphology in LOX cells with
predominantly monopolar spindle formations. Altogether, these results
propose an involvement of c-Rel in cell cycle regulation, thereby suggesting a
novel tumor-promoting role in human melanoma cells with putative
implications as a new combination target in melanoma therapy. | de |
| dc.contributor.coReferee | Oppermann, Martin Prof. Dr. | |
| dc.subject.eng | c-Rel | de |
| dc.subject.eng | NF-κB | de |
| dc.subject.eng | melanoma | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-21.11130/00-1735-0000-0005-12FE-9-3 | |
| dc.affiliation.institute | Medizinische Fakultät | de |
| dc.subject.gokfull | Medizin (PPN619874732) | de |
| dc.subject.gokfull | Dermatologie / Venerologie - Allgemein- und Gesamtdarstellungen (PPN619876174) | de |
| dc.description.embargoed | 2020-03-10 | |
| dc.identifier.ppn | 1688900500 | |