Einfluss von RhoA auf die Invasivität von Mammakarzinomzellen

Influence of RhoA on Inavasiveness of Breast Cancer Cells

by Franziska Schömel
Doctoral thesis
Date of Examination:2021-05-26
Date of issue:2021-05-20
Advisor:Prof. Dr. Carsten Gründker
Referee:Prof. Dr. Carsten Gründker
Referee:Prof. Dr. Steven Johnsen
Persistent Address: http://dx.doi.org/10.53846/goediss-8607

 

 

Files in this item

Name:1_updated_Promotion_Schömel,Franziska.pdf
Size:712.Kb
Format:PDF

The following license files are associated with this item:

Abstract

English

Breast cancer is the most frequent cancer women suffer from. The most common cause of death is metastasis not the tumor itself, in this stage the disease is no longer curable. This underlines the great significance of examining metastasis to understand it and to find possible therapeutic targets. In the present work it was explored which influence a knockdown of the Rho-GTPase protein RhoA has on non-invasive breast cancer cells MCF-7 and T47D. RhoA is well known for its contribution in stressfiber formation and formation of focal adhesions and therefore a non-motile phaenotype. siRNA-interference was used for the knockdown of RhoA and RT-qPCR and Western Blot to prove the success. With different co-culture assays we observed if the knockdown leads to a change in invasion of the cells. Additionally a proliferation assay was performed and the amount and quality of f-Aktin analysed. We found out that in MCF-7 the invasiveness increased significantly and consistently the amount of F-aktin decreased significantly after RhoA-knockdown. We assume that the reduced stressfiber formation after RhoA-knockdown is responsible for the increase of invasion. In T47D these effects could not be observed. The proliferation was not influenced by RhoA-knockdown. Finally the question is discussed if RhoA is a tumor suppressor or an oncogene and how it possibly switches between these different states.
Keywords: RhoA; breast cancer; invasion; MCF-7; T47D
 
Statistik