Anwendung der Fluoreszenz-Korrelations-Spektroskopie zur Untersuchung dynamischer Prozesse in lebenden Zellen

Jordan, Randolf

dc.contributor.advisor	Neher, Erwin Prof. Dr.	de
dc.contributor.author	Jordan, Randolf	de
dc.date.accessioned	2001-05-09T15:06:58Z	de
dc.date.accessioned	2013-01-18T10:35:38Z	de
dc.date.available	2013-01-30T23:51:05Z	de
dc.date.issued	2001-05-09	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0006-AE88-1	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-2112
dc.format.mimetype	ContentType:application/pdf Size:4966	de
dc.language.iso	ger	de
dc.rights.uri	http://webdoc.sub.gwdg.de/diss/copyrdiss.htm	de
dc.title	Anwendung der Fluoreszenz-Korrelations-Spektroskopie zur Untersuchung dynamischer Prozesse in lebenden Zellen	de
dc.type	doctoralThesis	de
dc.title.translated	Application of fluorescence correlation spectroscopy to investigate dynamic processes in living cells	de
dc.contributor.referee	Troe, Jürgen Prof. Dr.	de
dc.date.examination	2000-10-31	de
dc.subject.dnb	540 Chemie	de
dc.description.abstracteng	In this thesis the Fluorescence Correlation Spectroscopy (FCS) technique is applied to cell-physiologically relevant reaction and diffusion problems in living cells. In the nucleus as well as in the cytoplasm of bovine adrenal chromaffin cells the FCS method is used to determine diffusion coefficients of calcium chelators that are often used as calcium indicators. At the interface of hippocampal cell membrane and extracellular solution the specific molecular interaction of a dye-labelled benzodiazepine with its receptor is examined. The affinity of the ligand-receptor-complex as well as the reaction rates of the complex are determined. The applicability of different dyes for ligand-labelling in FCS experiments is investigated. The FCS technique is suited to resolve the dynamics of single synaptic vesicles in hippocampal nerve endings. The effect of kinases, phosphatases and the cytoskeleton on the vesicle mobility are investigated. The mobility of these vesicles in unstimulated boutons is mainly due to active transport and under the control of the myosin light chain kinase. Inhibition of the phosphatases 1 and 2A leads to an increased vesicle mobility which can be further increased by destruction of the actin filaments in the synapse.	de
dc.contributor.coReferee	Zeeck, Axel Prof. Dr.	de
dc.subject.topic	Mathematics and Computer Science	de
dc.subject.eng	fluorescence correlation spectroscopy	de
dc.subject.eng	hippocampal neuron	de
dc.subject.eng	synaptic vesicle	de
dc.subject.eng	synapse	de
dc.subject.eng	benzodiazepine	de
dc.subject.eng	receptor binding study	de
dc.subject.eng	diffusion coefficient	de
dc.subject.bk	35.22	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-1016-8	de
dc.identifier.purl	webdoc-1016	de
dc.affiliation.institute	Fakultät für Chemie	de
dc.subject.gokfull	SD	de
dc.identifier.ppn	330817736

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