Funktionelle Rekonstitution von Connexonen in artifizielle Membranen: Expression, Reinigung und Charakterisierung von Connexin 43

Carnarius, Christian

dc.contributor.advisor	Steinem, Claudia Prof. Dr.	de
dc.contributor.author	Carnarius, Christian	de
dc.date.accessioned	2012-11-19T15:44:04Z	de
dc.date.accessioned	2013-01-18T10:39:11Z	de
dc.date.available	2013-01-30T23:51:01Z	de
dc.date.issued	2012-11-19	de
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-000D-F053-F	de
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-2189
dc.format.mimetype	application/pdf	de
dc.language.iso	ger	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/	de
dc.title	Funktionelle Rekonstitution von Connexonen in artifizielle Membranen: Expression, Reinigung und Charakterisierung von Connexin 43	de
dc.type	doctoralThesis	de
dc.title.translated	Functional reconstitution of connexons in artificial membranes: expression, purification and characterization of connexin 43	de
dc.contributor.referee	Steinem, Claudia Prof. Dr.	de
dc.date.examination	2012-06-11	de
dc.subject.dnb	570 Biowissenschaften	de
dc.subject.dnb	Biologie	de
dc.subject.gok	SX 000	de
dc.subject.gok	WC 000	de
dc.subject.gok	WR 000	de
dc.subject.gok	SXH 000	de
dc.description.abstracteng	Connexin 43 (Cx43) is the most prominent member of the connexin family in mammalian tissues. In biological lipid membranes, six connexin subunits assemble into a hemichannel (connexon). Direct communication of apposing cells is realized by two adjacent hemichannels, forming gap junction channels. Membrane channel proteins are pivotal for communication between cells. Dysfunctional ion channels are associated to diseases like osteoporosis, epilepsy, ataxia, myotonia and cardiac arrhythmia. Therefore, there is an interest in studying these channel proteins to elucidate their functional properties and determine how to manipulate their functionality. This is commonly carried out by ion channel recordings, which require invasive contacting by voltage clamp methods in cells. The alternative approach taken here was to utilize connexon-doped artificial membranes for electrical coupling of connexon-expressing cells via gap junction channels. In this work, an expression system in Pichia pastoris to recombinantly produce and purify Cx43 as well as Cx43 fused to green fluorescent protein (Cx43	de
dc.contributor.coReferee	Griesinger, Christian Prof. Dr.	de
dc.subject.topic	Chemistry	de
dc.subject.ger	Connexin 43	de
dc.subject.ger	Cx43	de
dc.subject.ger	Grün fluoreszierendes Protein	de
dc.subject.ger	GFP	de
dc.subject.ger	Porenüberspannende Membranen	de
dc.subject.ger	Poröses Aluminiumoxid	de
dc.subject.ger	Artifizielle Membranen	de
dc.subject.ger	Planar Patch Clamp	de
dc.subject.ger	Melittin	de
dc.subject.ger	Pichia pastoris	de
dc.subject.ger	D. discoideum	de
dc.subject.eng	connexin 43	de
dc.subject.eng	Cx43	de
dc.subject.eng	green fluorescent protein	de
dc.subject.eng	GFP	de
dc.subject.eng	pore spanning membranes	de
dc.subject.eng	porous alumina	de
dc.subject.eng	artificial membranes	de
dc.subject.eng	planar patch clamp	de
dc.subject.eng	melittin	de
dc.subject.eng	Pichia pastoris	de
dc.subject.eng	Dictyostelium discoideum	de
dc.subject.bk	42.12	de
dc.subject.bk	35.78	de
dc.subject.bk	35.71	de
dc.identifier.urn	urn:nbn:de:gbv:7-webdoc-3797-1	de
dc.identifier.purl	webdoc-3797	de
dc.affiliation.institute	Fakultät für Chemie	de
dc.identifier.ppn	737898712	de

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Fakultät für Chemie (inkl. GAUSS) [701]

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