In vitro effects of periodontopathic bacteria on the proliferation and osteogenic potential of human mesenchymal stem cells

In vitro effects of periodontopathic bacteria on the proliferation and osteogenic potential of human mesenchymal stem cells

Ahmed Baligh

Doctoral thesis

Date of Examination: 2013-03-05

Date of issue: 2013-03-06

Advisor: Miosge, Nicolai Prof. Dr.

Referee: Miosge, Nicolai Prof. Dr.

Referee: Lüder, Carsten Prof. Dr.

Referee: Guan-Schmidt, Kaomei PD Dr.

Persistent Address: http://hdl.handle.net/11858/00-1735-0000-000E-0B5A-C

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Abstract

English

Periodontitis is a bacterial biofilm induced inflammatory disease that results in the destruction of the periodontal components including the destruction of the PDL, cementum, gingiva, and alveolar bone. It has been found that periodontal regeneration by conventional methods, for example, scaling, root planning, bone grafts, and barrier membranes might be effective in some cases. However, these conventional methods have shown some limitations with certain extensive cases. That is the main reason of the great concern about periodontal regeneration aided by the use of stem cells. This study mainly concentrates on the immortalized human mesenchymal stem cells (hMSCs), and their ability to withstand culturing and incubation with the bacteria that are involved in periodontitis, namely, Actinobacillus actinomycetemcomitans, and Eikenella corrodens, in order to elucidate their effects on the behavior of the hMSCs regarding their stemness, degree of osteogenic differentiation, and proliferation capacity.

Keywords: periodontitis; immortalized human mesenchymal stem cells; periodontopathic bacteria