Die Wirkung von Dihydrotestosteron, 17-ß-Östrogen, Genistein und Equol auf das Remodelling der defekten osteoporotischen Tibia der männlichen Ratte

The effect of Dihydrotestosteron, 17ßEstrogen, Genistein and Equol on the remodelling of the defect osteoporotic male rat tibia

by Philipp Kauffmann
Doctoral thesis
Date of Examination:2013-12-10
Date of issue:2013-12-02
Advisor:Prof. Dr. Dr. Karl Günter Wiese
Referee:Prof. Dr. Dr. Karl Günter Wiese
Referee:Dr. Mohammad Tezval
Persistent Address: http://dx.doi.org/10.53846/goediss-4199

 

 

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Abstract

English

Introduction: Osteoporosis was long considered to be women desease. Studies have shown the importance of further research on the area of male osteoporosis. The objective of our study was to show the effect of Dihydrotestosteron, 17-ß-Oestrogen, Genistein and Equol on the remodelling of the defect, osteoporotic male rat tibia. Material and Methods: 180 Sprague Dawley male rats were divided in 5 groups of 36 animals. One group was feeded with soja free (control group), one group with DHT, one group with E2, one group with GEN and one group with EQ. The rats were orchidectomied and after manifest osteoporosis a trepanation of the tibia was performed. Food Change started the Treatment. After a time period of 46, 53 and 102 days 12 rats of each group were sacrificed. Body weight was measured and histomorphometry was performed in order to analyse changes in bone structure under treatment. Results: The SF, GEN, EQ and DHT group gain weight whereas E2 loses weight. For the diaphyseal cortical bone a significant gain could be observed by DHT and E2. The phytohormons EQ and GEN had only a temporally positive effect on the diaphyseal cortical bone which could not been observed throughout the whole study period. Only E2 preserved trabecular bone temporally and lead to a significant smaller epiphyses. Conclusion: Especially the cortical bone was influenced. Phytohormons are only a short term therapy option in the male osteoporotic defect healing, they are not useful for a long term treatment. E2 and DHT preserve the the diaphyseal cortical bone. This effect is stronger in the E2 than in the DHT group. Furthermore testosteron which could not be converted by α-Aromatase into oestrogen is more suitable than DHT.
Keywords: bone remodelling; osteoporosis; dihydrotestosteron; estrogen; equol; genistein
 
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