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Untersuchung einzelner SNARE-vermittelter Membranfusionsereignisse auf planaren porenüberspannenden Membranen

dc.contributor.advisorSteinem, Claudia Prof. Dr.
dc.contributor.authorSchwenen, Lando Lantbert Gregor
dc.date.accessioned2015-10-20T09:13:28Z
dc.date.available2015-10-20T09:13:28Z
dc.date.issued2015-10-20
dc.identifier.urihttp://hdl.handle.net/11858/00-1735-0000-0023-9650-F
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-5308
dc.language.isodeude
dc.publisherNiedersächsische Staats- und Universitätsbibliothek Göttingende
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc540de
dc.titleUntersuchung einzelner SNARE-vermittelter Membranfusionsereignisse auf planaren porenüberspannenden Membranende
dc.typedoctoralThesisde
dc.title.translatedInvestigation of Single SNARE-mediated Membrane Fusion Events on Planar Pore-spanning Membranesde
dc.contributor.refereeSteinem, Claudia Prof. Dr.
dc.date.examination2015-06-04
dc.description.abstractengEven though there are a number of different in vitro fusion assays to analyze neuronal SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) mediated fusion, still the essential features of the in vivo situation are only partially captured. In this thesis an in vitro fusion assay that provides a planar solvent-free pore-spanning membrane containing a ternary acceptor SNARE complex, which mimics the presynaptic membrane, to monitor fusion of single synaptobrevin containing vesicles is presented. Protein-containing pore-spanning membranes were obtained by spreading of giant unilamellar vesicles on gold covered6-mercapto-1-hexanol functionalized porous siliconnitride substrates with pore diameters of 1.2µm. The mobility of Lipids and the SNAREs in the pore-spanning membranes was shown by FCS-experiments, which is prerequisite for the formation of active fusion complexes. The fusion process was highly specific with an efficiency of 50 % and was analyzed by two color confocal laser scanning fluorescence microscopy in a timeresolved manner allowing to distinguish between vesicle docking, hemifusion and full fusion. A kinetics analysis revealed that two reaction steps need to take place to progress to fusion with a lifetime of the docked vesicles of about 50s.de
dc.contributor.coRefereeSalditt, Tim Prof. Dr.
dc.subject.gerSNAREde
dc.subject.gerMembranfusionde
dc.subject.gerFusionsassayde
dc.subject.gerporenüberspannende Membrande
dc.subject.gerDiffusion in Lipidmembranende
dc.subject.gerkonfokale Laserrastermikroskopiede
dc.subject.gerHemifusionde
dc.subject.gerOberflächenfunktionalisierungde
dc.subject.gerporöse Substratede
dc.subject.gerLipidmembranende
dc.subject.gerVesikelfusionde
dc.subject.gerMobilität von SNARE-Proteinende
dc.subject.engSNAREde
dc.subject.engsoluble N-ethylmaleimide-sensitive factor attachment protein receptorde
dc.subject.engfusionde
dc.subject.engfusion assayde
dc.subject.engpore-spanning membranesde
dc.subject.engmobility of Lipidsde
dc.subject.engmobility of SNAREsde
dc.subject.engporous substratesde
dc.subject.engvesicle dockingde
dc.subject.enghemifusionde
dc.subject.engconfocal laser scanning fluorescence microscopyde
dc.subject.englipid membranesde
dc.subject.engsurface functionalizationde
dc.subject.engmembrane fusionde
dc.subject.engmembrane diffusionde
dc.identifier.urnurn:nbn:de:gbv:7-11858/00-1735-0000-0023-9650-F-2
dc.affiliation.instituteFakultät für Chemiede
dc.subject.gokfullChemie  (PPN62138352X)de
dc.identifier.ppn837584302


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