dc.contributor.advisor | Hagos, Yohannes Prof. Dr. | |
dc.contributor.author | Sendler, Mark Florian | |
dc.date.accessioned | 2015-11-25T09:49:52Z | |
dc.date.available | 2015-12-02T23:50:05Z | |
dc.date.issued | 2015-11-25 | |
dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-0028-8641-A | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-5381 | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-5381 | |
dc.language.iso | deu | de |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.ddc | 610 | de |
dc.title | Klonierung und funktionelle Charakterisierung des pOAT1 in ok-Zellen | de |
dc.type | doctoralThesis | de |
dc.title.translated | Cloning and functional characterization of the pOAT1 in ok-cells | de |
dc.contributor.referee | Bohnsack, Markus Prof. Dr. | |
dc.date.examination | 2015-11-25 | |
dc.description.abstracteng | We created and screened a pig kidney cDNA library for the organic anionen transporter 1 (pOAT1) und could isolate two splice variants (pOAT1: EMBL Acc. AJ308234 and pOAT1A: AJ308235). The functional characterization by expression of pOAT1 in Xenopus laevis oocytes and ok-cells showed an [3H]PAH uptake only for pOAT1. The apparrent Km for [3H]PAH in oocytes is 3,75 ± 1,6 µM and in ok-cells it is 8,54 ± 1,2 µM. This uptake was inhibited by 1mM probenecid and 1mM glutarate. The functional characterization revealed an similar cis inhibition of the pOAT1 mediated uptake for [3H]PAH by pyrazionat, urate, glutarate and probenicid as in pig BLMV. We also could show that the chlorid dependent uptake of [3H]PAH in pig BLMV is mediated by the pOAT1 whereas the chlorid independent urate uptake is mediated by the pOAT3. Testing steroids as substrate for the pOAT1 demonstrated a strong inhibition of the [3H]PAH uptake (Ki for estrone sulfate 113± 4,9µM; Ki for DHEA sulfate 116,5± 3,5µM) whereas [3H]estrone sulfate was not transported by the pOAT1. | de |
dc.contributor.coReferee | Schön, Margarete Prof. Dr. | |
dc.subject.ger | pOAT1 | de |
dc.subject.ger | organische Anionen Transporter | de |
dc.subject.ger | ok-Zellen | de |
dc.subject.ger | slc22 Familie | de |
dc.subject.ger | Schwein | de |
dc.subject.eng | pOAT1 | de |
dc.subject.eng | organic anion transporter | de |
dc.subject.eng | ok cells | de |
dc.subject.eng | slc22 family | de |
dc.subject.eng | pig | de |
dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-0028-8641-A-6 | |
dc.affiliation.institute | Medizinische Fakultät | de |
dc.subject.gokfull | Medizin (PPN619874732) | de |
dc.description.embargoed | 2015-12-02 | |
dc.identifier.ppn | 840882491 | |