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dc.contributor.advisor Rizzoli, Silvio Prof. Dr.
dc.contributor.author Zhang, William
dc.date.accessioned 2016-03-31T08:35:59Z
dc.date.available 2016-03-31T08:35:59Z
dc.date.issued 2016-03-31
dc.identifier.uri http://hdl.handle.net/11858/00-1735-0000-0028-8710-0
dc.language.iso eng de
dc.relation.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc 570 de
dc.title Novel Applications of Super-Resolution Microscopy in Molecular Biology and Medical Diagnostics de
dc.type doctoralThesis de
dc.contributor.referee Outeiro, Tiago Fleming Prof. Dr.
dc.date.examination 2015-11-18
dc.description.abstracteng Despite recent advances in microscopy techniques, the underlying labeling assays remained mostly unchanged. However, super-resolution techniques require other staining conditions than conventional light microscopy, like more dense labeling, which is not always trivial to achieve. In this work, I present two novel applications of stimulated emission depletion (STED) microscopy to the fields of molecular biology and medical diagnostics. For one, I established a fluorescent in situ hybridization (FISH) protocol to investigate mRNAs of the three synaptic proteins synaptophysin, synaptobrevin, and synaptotagmin in primary hippocampal neurons. This achieved more precise information regarding the mRNA numbers and organization than conventional confocal microscopy. Secondly, I applied STED microscopy for the development of a novel diagnosis method for Alzheimer’s disease (AD). The increased resolution was sufficiently high to discriminate low and high molecular weight β-amyloid (Aβ) aggregates produced in vitro. Analysis of cerebrospinal fluid (CSF) samples from 36 AD patients, 11 patients with mild cognitive impairment (MCI), and 21 controls allowed a separation of AD patients from controls with ~87% specificity and ~79% sensitivity. In conclusion, this work illustrates the need for optimization of long established methods depending on the imaging technique and sample to obtain more accurate data. de
dc.contributor.coReferee Braus, Gerhard Prof. Dr.
dc.subject.eng super-resolution de
dc.subject.eng Alzheimer's disease de
dc.subject.eng fluorescence in situ hybridization de
dc.identifier.urn urn:nbn:de:gbv:7-11858/00-1735-0000-0028-8710-0-0
dc.affiliation.institute Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) de
dc.subject.gokfull Biologie (PPN619462639) de
dc.identifier.ppn 855865261

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