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dc.contributor.advisor Neumann, Heinz Prof. Dr.
dc.contributor.author Rall, Nils Arne
dc.date.accessioned 2016-04-13T08:34:47Z
dc.date.available 2016-04-13T08:34:47Z
dc.date.issued 2016-04-13
dc.identifier.uri http://hdl.handle.net/11858/00-1735-0000-0028-872D-1
dc.language.iso deu de
dc.relation.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc 572 de
dc.title A Method for the Quantitative Analysis of Protein-Protein Interactions In Vivo en
dc.type doctoralThesis de
dc.contributor.referee Neumann, Heinz Prof. Dr.
dc.date.examination 2016-03-22
dc.description.abstracteng The function and activity of proteins is often modulated by other proteins they interact with. To understand cellular behaviour at the system level, a complete description of interactions among different proteins and the dynamic nature of these interactions is required.  Identification and quantification of proteins based on measured peptide intensities represents the highest standard for an unbiased and definite determination of protein interaction partners.  Inspired by the display of effectiveness of pBPA in vivo crosslinking in elucidating the mechanistic details of chromatin condensation in S. cerevisiae (Wilkins et al., 2014), the research objective of this PhD thesis was the development of a method for the quantitative identification of histone interaction partners for further decoding of chromatin structure dynamics. First, the effectiveness of pBPA in vivo crosslinking for capturing histone-protein interactions in living yeast cells was successfully confirmed. Secondly, an immunoprecipitation protocol for pulldown of HA-tagged histone pBPA mutants and their UV-induced crosslink products was established and adapted to SILAC conditions. Thirdly, initial SILAC analyses were performed and optimized until reproducible results of increased quality were obtained. Optimization led to the identification of numerous chromatin-related proteins, including a confirmed interaction partner.  de
dc.contributor.coReferee Urlaub, Henning Prof. Dr.
dc.subject.eng Genetic code expansion de
dc.subject.eng SILAC de
dc.subject.eng Mass spectrometry de
dc.subject.eng Chromatin de
dc.subject.eng Histones de
dc.subject.eng Crosslinking de
dc.subject.eng In vivo de
dc.identifier.urn urn:nbn:de:gbv:7-11858/00-1735-0000-0028-872D-1-5
dc.affiliation.institute Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) de
dc.subject.gokfull Biologie (PPN619462639) de
dc.identifier.ppn 857081365

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