| dc.contributor.advisor | Schild, Detlev Prof. Dr. Dr. | |
| dc.contributor.author | de Jong, Daniëlle | |
| dc.date.accessioned | 2016-08-30T08:51:38Z | |
| dc.date.available | 2016-08-30T08:51:38Z | |
| dc.date.issued | 2016-08-30 | |
| dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-0028-881C-0 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-5835 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-5835 | |
| dc.language.iso | eng | de |
| dc.relation.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject.ddc | 570 | de |
| dc.title | Glomerular information processing in Xenopus laevis | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Stühmer, Walter Prof. Dr. | |
| dc.date.examination | 2016-04-22 | |
| dc.description.abstracteng | Olfactory glomeruli are structural and functional neuropils in the olfactory bulb. Although
many olfactory receptor neurons (ORNs) project to one glomerulus, stimulusinduced
responses within single glomeruli could not be previously distinguished using
calcium imaging. At the level of the olfactory epithelium, however, activity differences
within ORN subpopulations have been reported.
I studied stimulus-induced and spontaneous activity patterns of intraglomerular ORN
axons in order to obtain a deeper understanding of presynaptic odor representations
within single glomeruli. Dose-response profiles and spontaneous activity patterns were
obtained for glomeruli stained with CaGreen or JG205, which is a newly synthesized
dye. From CaGreen-stained glomeruli odorant response profiles were acquired as well.
Activity-specific information was then used to investigate how individual intraglomerular
axons can be distinguished based on activity patterns.
The presented data revealed, that, a combination of fast 3D line-illumination microscopy
and activity correlation of spontaneous activity patterns was sufficient for
the visualization of JG205-stained ORN axons. An activity-based separation of intraglomerular
axons was, however, only partially possible for axons stained with CaGreen.
The latter confirms previously reported results, namely, that response profiles and/or
spontaneous activities from CaGreen-stained glomeruli cannot be assigned to individual
CaGreen-stained axons (Wachowiak et al., 2004). The study of information processing within individual intraglomerular axons is essential
for a deeper understanding of the presynaptic representation of odors. Visualizing
and identifying individual intraglomerular axons simultaneously based on their activity
patterns facilitates the study of presynaptic information processing. Furthermore,
the combination of fast 3D calcium imaging and activity correlation imaging can also
be used to investigate information processing in single neurons across brain areas and
species. | de |
| dc.contributor.coReferee | Müller, Michael Prof. Dr. | |
| dc.subject.eng | olfactory glomeruli | de |
| dc.subject.eng | glomerular response profiles | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-0028-881C-0-7 | |
| dc.affiliation.institute | Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) | de |
| dc.subject.gokfull | Biologie (PPN619462639) | de |
| dc.identifier.ppn | 869469541 | |