• Deutsch
    • English
  • English 
    • Deutsch
    • English
  • Login
Item View 
  •   Home
  • Zentren & Graduiertenschulen
  • GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften
  • Item View
  •   Home
  • Zentren & Graduiertenschulen
  • GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften
  • Item View
JavaScript is disabled for your browser. Some features of this site may not work without it.

Characterization of the retinoic acid-induced gene network responsible for pancreas specification in Xenopus laevis

by Maja Gere
Doctoral thesis
Date of Examination:2016-03-21
Date of issue:2017-02-06
Advisor:Prof. Dr. Tomas Pieler
Referee:Prof. Dr. Herbert Jäckle
Referee:Prof. Dr. Andreas Wodarz
Referee:Prof. Dr. Ahmed Mansouri
Referee:Prof. Dr. Ernst A. Wimmer
Referee:Prof. Dr. Matthias Dobbelstein
crossref-logoPersistent Address: http://dx.doi.org/10.53846/goediss-6108

 

 

Files in this item

Name:Maja Gere PhD thesis SUB.pdf
Size:5.52Mb
Format:PDF
ViewOpen

The following license files are associated with this item:


Abstract

English

Retinoic acid (RA) is critically required for pancreas specification in Xenopus and other vertebrates. However, the gene network that is directly induced by RA-signaling in this context remains to be defined. We identified 22 RA-target genes through RNA-sequencing of in vitro generated pancreatic organoids. One of these is Hnf1b, which has been shown to be associated with a monogenic form of diabetes in humans and with pancreas hypoplasia in vertebrates. Functional analyses of Hnf1b in pancreatic organoids and whole Xenopus embryos revealed its early requirement for pancreatic progenitor formation. However, we also found that Hnf1b alone is not sufficient to substitute for RA in pancreas specification, indicating a requirement of one or more additional RA-responsive activities. Furthermore, we identified the Wnt-receptor Fzd4 as direct RA-target and novel regulator in pancreas development. Loss-of-function experiments in pancreatic organoids reveal a role of this Wnt-signaling component in pancreas development. Additional experimental data suggest that a modulation of non-canonical Wnt-signaling activity by RA, probably mediated through Fzd4, is required for a proper pancreas specification.
Keywords: pancreas; retinoic acid
 

Statistik

Publish here

Browse

All of eDissFaculties & ProgramsIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesTypeThis FacultyIssue DateAuthorAdvisor & RefereeAdvisorRefereeTitlesType

Help & Info

Publishing on eDissPDF GuideTerms of ContractFAQ

Contact Us | Impressum | Cookie Consents | Data Protection Information
eDiss Office - SUB Göttingen (Central Library)
Platz der Göttinger Sieben 1
Mo - Fr 10:00 – 12:00 h


Tel.: +49 (0)551 39-27809 (general inquiries)
Tel.: +49 (0)551 39-28655 (open access/parallel publications)
ediss_AT_sub.uni-goettingen.de
[Please replace "_AT_" with the "@" sign when using our email adresses.]
Göttingen State and University Library | Göttingen University
Medicine Library (Doctoral candidates of medicine only)
Robert-Koch-Str. 40
Mon – Fri 8:00 – 24:00 h
Sat - Sun 8:00 – 22:00 h
Holidays 10:00 – 20:00 h
Tel.: +49 551 39-8395 (general inquiries)
Tel.: +49 (0)551 39-28655 (open access/parallel publications)
bbmed_AT_sub.uni-goettingen.de
[Please replace "_AT_" with the "@" sign when using our email adresses.]