Dokumente GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften nach Gutachter "Urlaub, Henning Prof. Dr."
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Investigation of early assembly of OXPHOS complexes during mitochondrial translation
(2020-12-02)After a long time of coevolution between mitochondria and their eukaryotic host cells, the mitochondrial genome has diverged severely from it’s α- proteobacterial ancestors. Mitochondrial genes have mainly been transferred to ... -
Investigation of protein-RNA interactions by UV cross-linking and mass spectrometry: methodological improvements toward in vivo applications
(2014-04-07)Protein–RNA complexes play key roles in a variety of cellular functions such as gene expression and its regulation. One frequently applied method for the investigation of direct protein–RNA interactions is UV induced ... -
Kinetic Analysis of Mammalian Translation Initiation
(2021-01-22)During translation initiation in mammalian cells, 80S ribosomes are assembled at the translation start site on mRNA via a cap-dependent scanning mechanism. The formation of the 80S initiation complex (IC) is essential for ... -
Localisation and function of Slam in the early Drosophila embryo
(2014-11-06)Embryogenesis of many insects starts with a syncytial stage characterised by 13 rapid nuclear divisions. During the following interphase, the plasma membrane of the embryo invaginates between each nucleus to give rise to ... -
Mechanism of non-canonical ubiquitination by ubiquitin conjugating enzyme Ubc6
(2023-10-18)Protein quality control by ubiquitination occurs due to the sequential action of three enzyme classes: ubiquitin-activating (E1), ubiquitin-conjugating (E2) and ubiquitin-ligases (E3). Conventionally, ubiquitin (Ub) ... -
A Method for the Quantitative Analysis of Protein-Protein Interactions In Vivo
(2016-04-13)The function and activity of proteins is often modulated by other proteins they interact with. To understand cellular behaviour at the system level, a complete description of interactions among different proteins and the ... -
Molecular insights into the roles of RNA helicases during large ribosomal subunit assembly
(2021-02-25)RNA helicases are enzymes present in all domains of life that are important in many aspects of RNA metabolism. They harbor a conserved helicase core that uses the energy of NTP binding and hydrolysis to change affinity to ... -
Molecular insights into the Tau-actin interaction
(2018-05-04)Tau protein is classically considered as a neuronal microtubule-associated protein that stabilizes microtubules and supports the outgrowth of axons. The protein can modulate the transport of vesicles and organelles along ... -
MS-based quantitative analysis of the CRM1 export pathway and spatial proteomics of the Xenopus laevis oocyte
(2015-09-22)Nuclear export is a vital cellular process, which supplies the cytoplasm with essential biological macromolecules, such as matured tRNAs and ribosomal subunits. This process is carried out by a set of proteins called ... -
NMR Spectroscopy of the Tau-Microtubule Interaction
(2015-12-10)Tau is an intrinsically disordered, microtubule associated protein and is responsible for the promotion of microtubule (MT) formation and stabilization. The human Tau protein has six different isoforms, which are expressed ... -
Novel export and import pathways in S. cerevisiae identified by an engineered SUMO system
(2018-09-07)Nuclear transport receptors (NTRs) mediate the translocation of cargos between the cytoplasm and the nucleus. NTRs are classified either as importins or exportins according to the directionality of the cargo transport. To ... -
Nucleoporin-binding nanobodies that either track or trap uclear pore complex assembly
(2021-03-25)Nuclear pore complexes (NPCs) are large multi-protein assemblies that control the transport of macromolecules between the nucleus and the cytoplasm. Their formation from ~ 1,000 individual polypeptides is an impressive ... -
Post-Translational Protein Modifications involved in Exo- and Endocytosis of Synaptic Vesicles
(2021-10-26)Neurotransmitter release is a key step that enables information flow between the pre- and post-synapse. However, regulation of the neurotransmitter release remains an intricate and widely unexplored matter despite recent ... -
Protein Interactions in minimal cells
(2022-05-18)Minimal cells are used to model and understand life’s complex processes. The most essential processes of life are thought to include cell replication, energy metabolism and genome maintenance. However, construction of a ... -
Purification of organellar macromolecular complexes for structural biology
(2022-03-14)Photosynthesis is the source of life on Earth. In oxygenic photosynthesis, water and carbon dioxide are converted into organic matter using of solar energy that results in the release of oxygen. PSII, a supra-molecular ... -
Quantitative Modeling of RNA-Protein Interactions
(2021-05-07)RNA-binding proteins (RBPs) impact every aspect of RNA metabolism including RNA transcription, maturation, export, localization, translation, and stability. Specific RNA-protein interactions therefore play a central role ... -
Quantitative phosphoproteomics for studying B-cell receptor signaling in Burkitt’s lymphoma
(2017-02-16)Protein phosphorylation plays an important role in transmitting cellular signals. Phosphorylation has been mostly characterized by hypothesis-driven and low-throughput approaches in the past. However, novel phosphorylation ... -
Recognition Elements for Elongation Factor P on the Ribosome
(2019-01-25)Translation elongation is a multi-step process orchestrated by elongation factors. Elongation factors G and Tu are required for each round of translation elongation, whereas elongation factor P is only required to assist ... -
Reconstitution of Doa10-mediated ER-associated protein degradation with purified components
(2020-11-09)ER-associated protein degradation (ERAD) is a protein quality control pathway at the ER membrane mediated by membrane-embedded ubiquitin ligase complexes. During ERAD, membrane and luminal proteins are ubiquitinated and ... -
Reconstitution of retrotranslocation by the Hrd1 ubiquitin ligase with purified components
(2020-03-11)In eukaryotic organisms, a large fraction of newly-synthesized proteins are destined for the endomembrane system or for secretion. The entry gate for these proteins is the endoplasmic reticulum (ER). Proteins are imported ...