Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Advisor "Cramer, Patrick Prof. Dr."
Now showing items 1-14 of 14
-
Structural and biochemical studies on co-transcriptional endonuclease reactions
(2025-03-25)Influenza, an acute respiratory disease, causes 290,000 to 650,000 human deaths annually (Iuliano et al., 2018; WHO, 2023). During an influenza infection, the virus ensures proper handling of viral mRNA, including nuclear ... -
Uncovering the mode of action of small molecule inhibitors against the RNA polymerase of SARS-CoV-2
(2025-03-07)Severe acute respiratory syndrome coronavirus 2 (SARS CoV 2) is the causative agent of coronavirus disease 2019 (COVID-19). The pandemic has claimed more than 7 million lives since its outbreak at the beginning of 2020, ... -
Structural and biochemical studies on chromatin-protein complexes in transcription initiation
(2024-04-02)Gene expression is an essential process in the cell for the development and homeostasis of any organism. The eukaryotic genome is tightly packed with nucleosomes, the basic unit of chromatin, which protects DNA integrity. ... -
Structural and functional studies of RNA polymerase II passage through nucleosomes
(2024-03-05)Transcription by RNA polymerase II (Pol II) is an essential process of life occurring in the context of the compact eukaryotic nucleus. While transcription elongation has been extensively studied on linear DNA in vitro, ... -
Multi-omics studies of transcription regulatory mechanisms during human cell fate transition
(2024-02-16)RNA polymerase II (Pol II) transcription is an essential process in living organisms that requires accurate regulation and coordination in order to produce protein-coding and many types of non-coding transcripts. One of ... -
Structural studies of RNA polymerase II complexes with human TFIIS and capping enzymes
(2023-12-14)Transcription is a conserved fundamental cellular process carried out by RNA polymerases (Pol) that results in the synthesis of an RNA transcript. Transcription of protein coding genes by Pol II is the first step in ... -
Bioinformatics analysis of multi-omics data elucidates the primary function of Oct4 in gene regulation and pluripotency maintenance
(2022-03-14)The transcription factor (TF) Oct4 is essential for maintaining pluripotency in vitro as well as in vivo. The crucial function of Oct4 in control of pluripotency has been extensively studied during reprogramming of somatic ... -
Bioinformatics analysis of multi-omics data elucidates U2 snRNP function in transcription
(2021-08-16)Transcription by RNA polymerase II (Pol II) is an important step in cell function and regulation. Pol II transcription has been shown to be coupled to pre-mRNA splicing, but the underlying mechanisms remain poorly ... -
Structure-function analysis of human nucleotide excision DNA repair
(2021-04-09)An average human cell accumulates tens of thousands of DNA lesions per day. Therefore, DNA repair pathways are set up to continuously proofread the DNA and correct DNA lesions, thus ensuring accurate expression and ... -
Investigating phase separation mechanisms for transcriptional control
(2020-11-13)Transcription of protein-coding genes by RNA polymerase (Pol) II is a highly coordinated process. In metazoan cells, transcription is regulated both at the initiation step by recruitment of the Pol II machinery as well as ... -
The Mediator kinase module: structural and functional studies in transcription regulation
(2020-10-15)Transcription of protein-coding genes occurs when RNA polymerase II (RNAPII) and general transcription factors (GTFs) assemble at genomic promoter regions, forming a transcription pre-initiation complex (PIC), which ... -
Structure of mammalian RNA polymerase II elongation complex bound by α-amanitin and study of mammalian transcription termination and 3’ end processing
(2019-12-04)Chapter 1 RNA polymerase II (Pol II) is the central enzyme that transcribes eukaryotic protein-coding genes to produce mRNA. The mushroom toxin α-amanitin binds Pol II and inhibits transcription at the step of RNA chain ... -
Multi-omics analysis of transcription kinetics in human cells
(2019-08-01)Transcription of the eukaryotic genome is a highly regulated process which is accomplished in a number of steps, known as the transcription cycle. One of these steps is promoter-proximal pausing, a regulatory halt of the ... -
Structural and Functional Investigation of Promoter Distortion and Opening in the RNA Polymerase II Cleft
(2019-02-11)Transcription of genes is a central process of life that defines how genetic information is used. This determines the metabolic state, morphology and fate of a cell, tissue, organ and the entire organism. In eukaryotes, ...