Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Advisor "Ficner, Ralf Prof. Dr."
Now showing items 1-11 of 11
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UV and chemical crosslinking mass spectrometry for the analysis of protein-nucleic acid interactions
(2024-07-12)Protein-nucleic acid interactions are a key part of essential cellular processes and their disturbance often results in the development of diseases. Crosslinking mass spectrometry (XL-MS)-based approaches have become ... -
Structural and functional investigation of tRNA guanine transglycosylase
(2023-11-17)In this thesis, two novel structures were presented, jointly illuminating dimerization and tRNA binding by human TGT. A first structure, obtained by X-ray crystallography, allowed to give a thorough account of the atomic ... -
Functional and Structural Characterization of the DEAH-box Helicase Prp43
(2022-11-24)RNA helicases of the DEAH-box family fulfill essential functions in various aspects of RNA metabolism by reorganizing structured RNAs and RNPs through ATP-dependent motility in 3’ to 5’ direction along single-strands. Prp43 ... -
Structural and functional characterisation of tRNA modifying enzymes
(2021-09-10)Posttranscriptional and posttranslational modifications are key regulatory mechanisms to expand biological properties of proteins and nucleic acids in living cells. A tremendous number of chemical modifications is found ... -
Structure Based Search for Novel Nuclear Export Inhibiting Anti-Tumor Drugs
(2020-07-28)The intracellular distribution of proteins and ribonucleoproteins is a fundamental process in all eukaryotes that is critically vital for the normal functionality of the eukaryotic cell. In the last decades, it has been ... -
Biophysical and Crystallographic Characterization of Spliceosomal DExD/H-box ATPases
(2020-04-23)DEAH-box ATPases play a vital role in the activation, catalytic and disassembly steps of the spliceosome. They are key for the orchestration of conformational and compositional rearrangements during the splicing cycle. It ... -
Structural and Biochemical Investigation of tRNA Modifying Enzymes
(2018-11-02)Posttranslational and posttranscriptional modifications have long been known to modulate and extend the properties of proteins and nucleic acids. A vast variety of non-canonical bases have been found on RNA molecules, with ... -
Crystallization and biophysical characterization of spliceosomal protein complexes
(2015-02-20)mRNA splicing is a highly dynamic process by which non-coding sequences are removed from a pre-mature mRNA. It is catalyzed by the spliceosome, a huge and highly dynamic ribonucleoprotein complex consisting of up to five ... -
Structural and functional studies of eukaryotic translation initiation factors
(2014-08-19)Protein synthesis can be divided into three distinct subprocesses, namely initiation, elongation and termination. During the initiation phase, the elongation competent ribosome, which harbors the initiator tRNA in its ... -
Insights into the activation of the spliceosomal helicase Prp43
(2013-11-20)The excision of non-coding sequences that are present in the pre-mRNA is a hallmark of eukaryotic cells. This process is termed as splicing and is catalysed by the spliceosome, a single-turnover enzyme which consists of ... -
Structural and functional investigation of the protein synthesis in saccharomyces cerevisiae
(2012-02-24)Protein synthesis is an important house-keeping activity of all the cells. It follows the same concepts in all three domains of life: the genetic code is deciphered and transcribed into the ...