Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Advisor "Jakobs, Stefan Prof. Dr."
Now showing items 1-10 of 10
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PUCK: Primer Utilised CRISPR/Cas Knock-Ins for High Throughput Super-Resolution Microscopy
(2024-11-29)The insertion of tags into proteins via gene editing is a powerful tool for microscopy, enabling the comparative analysis of different proteins. The advent of CRISPR/Cas systems has greatly simplified gene editing and ... -
Depletion of respiratory chain complexes modulates cristae architecture via MIC10 in human cells
(2024-10-18)The complex structure of the inner mitochondrial membrane plays a key role in mitochondrial function. The inner mitochondrial membrane is divided into the inner boundary membrane and the cristae membrane, which is characterized ... -
Investigation of the Mitochondria-ER axis and its interconnection with the MICOS complex
(2024-09-19)Mitochondria-ER contact sites (MERCS) are essential cellular hubs for the exchange of signaling molecules, lipids and metabolites. A multitude of MERCS-associated proteins have been described in mammalian cells, and rising ... -
Investigation of OPA1 in Mitochondrial Dynamics and Ultrastructure
(2024-03-21)Mitochondria are highly dynamic organelles with a complex ultrastructure. The mitochondrial inner membrane (MIM) is folded into cristae in which oxidative phosphorylation, a process to generate energy for the cell, takes ... -
Automated STED microscopy for cell-biological high-throughput assays
(2022-11-28)In the past decade, super-resolution fluorescence microscopy has revolutionized biological research by enabling the study of biological functions down to the molecular scale. Despite profound technological developments, ... -
Mitochondrial adaptation at the neuronal presynapse
(2021-11-22)Synaptic transmission poses a major energy consuming process in the brain, but how neurons maintain a constant energy supply during extended synaptic activity and how presynaptic mitochondria contribute to the energy ... -
Engineering of a NIR fluorescent protein for live-cell nanoscopy
(2020-09-22)Since its first use as a genetically encoded fluorescent marker in 1994, GFP and its homologues have fundamentally revolutionized live-cell fluorescence imaging and became an essential tool for biomedical research. Many ... -
Development and characterization of novel reversibly switchable red fluorescent proteins with opposing switching modes
(2020-03-03)Since the discovery of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria, a multitude of fluorescent proteins have been derived from the original GFP and its homologs by protein engineering. Their ... -
Analysis of mitochondrial transcription and replication on the single nucleoid level
(2019-03-13)Human mitochondrial DNA (mtDNA) is a circular molecule encoding for 13 subunits of the respiratory chain that are essential for its maintenance. Inside the mitochondria, mtDNA is associated with various proteins, forming ... -
CRISPR-Cas9-mediated protein tagging in human cells for RESOLFT nanoscopy and the analysis of mitochondrial prohibitins
(2016-12-13)Ectopic overexpression of fluorescent fusion proteins for live cell imaging studies often leads to a multitude of artefacts, but protein expression at endogenous levels in mammalian cells was difficult to achieve so far. ...