Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Advisor "Rizzoli, Silvio Prof. Dr."
Now showing items 1-9 of 9
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The Dynamic Organization of Proteins in the Synaptic Bouton
(2022-09-12)Protein availability is crucial for maintaining cellular processes. In synapses, the identities and functions of proteins involved in neurotransmission are well known. Their spatial distribution, copy numbers, and lifetimes ... -
Optimization of tools for multiplexed super resolution imaging of the synapse
(2020-07-09)The synapse is the major site of neurotransmission in the brain. Even though the synapse has been extensively studied, artefact-free imaging tools are still necessary for its correct investigation. With the resolution of ... -
Long-Term Temporal Dynamics of Synaptic Vesicles
(2017-09-11)Neurotransmission requires the release of neurotransmitters from synaptic vesicles. This occurs via fusion of the vesicle to the pre-synaptic membrane upon stimulation. However, not all synaptic vesicles are equally ... -
Novel Applications of Super-Resolution Microscopy in Molecular Biology and Medical Diagnostics
(2016-03-31)Despite recent advances in microscopy techniques, the underlying labeling assays remained mostly unchanged. However, super-resolution techniques require other staining conditions than conventional light microscopy, like ... -
Non-Canonical Amino Acids as Minimal Tags for Investigating Protein Organization and Turnover
(2016-01-08)The burgeoning field of genetic code expansion provides new tools for specifically labeling proteins for a variety of applications. Clickable non-canonical amino acids (ncAA) have been refined for almost-quantitative and ... -
A novel membrane-binding probe for the morphological and molecular characterization of synaptic vesicle recycling pathways
(2015-05-12)An important anatomical feature of neuronal synapses is the presynaptic bouton, a structure that isolates synaptic vesicle recycling from constitutive membrane trafficking pathways. However, in some neurotransmitter-releasing ... -
Studying Protein Organization in Cellular Membranes by High-Resolution Microscopy
(2014-10-09)Most membrane proteins are found in clusters in the plasma membrane. For abundant proteins it is apparent that the clusters also tend to have patterned distributions, rather than being randomly scattered. To reveal the ... -
Synaptic vesicle recycling investigated by high-resolution microscopy in a conventional and a sensory synapse
(2012-10-02)Synaptic neurotransmission depends on the action of highly specialized small secretory organelles – synaptic vesicles. At all chemical synapses the synaptic vesicles release in a strictly regulated fashion their neurotransmitter ... -
Investigation of Endosomal Recycling of Synaptic Vesicles
(2010-11-05)During communication at chemical synapses, small neurotransmitter-filled synaptic vesicles, housed in the pre-synaptic nerve terminal (bouton), fuse with the plasma membrane (exocytosis) ...