Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Bohnsack, Markus Prof. Dr."
Now showing items 1-20 of 26
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Molecular mechanisms and cellular functions of RNA methyltransferases targeting non-coding RNAs
(2024-12-23)Cellular RNAs contain diverse modified nucleotides that regulate their structure, stability, biogenesis and molecular functions. In abundant non-coding RNAs such as ribosomal (r)RNAs, transfer (t)RNAs and small nuclear ... -
Mechanisms of chromosome segregation in mammalian oocytes
(2024-12-10)Errors in chromosome segregation in human females often result in eggs with the wrong number of chromosomes, a condition known as aneuploidy. The rate of aneuploidy increases dramatically in women with age, particularly ... -
Dynamics and regulation of the mitochondrial translation machinery
(2024-11-15)Throughout this project, we have successfully established and implemented a ribosome profiling protocol tailored for examination of translating mitochondrial ribosomes, thereby affording a deeper understanding of mitochondrial ... -
Mechanisms underlying age-related decline in mammalian female fertility
(2024-03-05)Reproductive longevity is crucial for fertility and impacts healthy ageing. Unlike in men, fertility in women starts to decline several decades before other signs of ageing set in. Female fertility is restricted by a limited ... -
Structural and functional investigation of tRNA guanine transglycosylase
(2023-11-17)In this thesis, two novel structures were presented, jointly illuminating dimerization and tRNA binding by human TGT. A first structure, obtained by X-ray crystallography, allowed to give a thorough account of the atomic ... -
Analysis of multifunctional RNA helicase regulation by protein cofactors in different gene expression processes
(2023-09-11)The biogenesis of ribonucleoprotein (RNP) complexes is a fundamental process in the gene expression network. Assembly of eukaryotic ribosomes and spliceosomes is highly orchestrated and entails the formation of numerous ... -
Functional characterisation of DHX proteins in the regulation of RNA metabolism and genome stability
(2023-08-08)Cells contain a multitude of different RNAs, which are highly versatile biomolecules that have crucial functions in almost all cellular processes. Furthermore, the inter-relationship between RNA and proteins is essential, ... -
Elongation factor P: mechanism of action and opportunities for drug design
(2023-07-24)EF-P is a conserved protein that has shape similarity to the tRNAs and binds to the E site of the ribosome. EF-P enhances the rate of peptide bond formation during the synthesis of polyPro sequences. In E. coli one third ... -
Translation Termination in Human Mitochondria: The Role of mtRF1 and mtRF1a
(2022-12-09)Translation termination is the last step of ribosomal protein synthesis. Dedicated translation factors are imported into mitochondria to convert the information stored in the retained mitochondrial DNA (mtDNA) and to ... -
Non-canonical functions of the MDM2 oncoprotein
(2022-11-04)The tumour suppressor gene TP53 is mutated in ~50% of human cancers. The p53 protein is a transcription factor which is induced by several cellular stresses, such as ribosomal stress and DNA damage. Expression of its target ... -
Actin promotes chromosome aggregation and accurate chromosome segregation in mammalian oocytes
(2022-09-15)Accurate chromosome segregation during meiosis is essential for the formation of a new life. During meiosis, spindle microtubules bind to the chromosomes’ kinetochores and arms in order to separate the genetic material. ... -
Chromatin reorganization, signaling responses and non-coding RNA regulators in homeostasis and senescence
(2022-04-21)In mammalian cells, a relatively limited fraction of genetic information is utilized in a variety of ways to give rise to distinct cellular phenotypes. Thus, the genotype essentially acts as input for multilayered regulatory ... -
Structural and functional characterisation of tRNA modifying enzymes
(2021-09-10)Posttranscriptional and posttranslational modifications are key regulatory mechanisms to expand biological properties of proteins and nucleic acids in living cells. A tremendous number of chemical modifications is found ... -
Mechanism of Germ Cell Specification in Early Zebrafish Embryogenesis
(2021-07-19)Reproduction is an essential feature of life that ensures the perpetuation and survival in many multicellular organisms while passing the genetic information to the next generation. The zebrafish germline is specified ... -
Structural investigation of functional nucleic acids
(2021-06-30)DNA enzymes, also known as deoxyribozymes, are synthetic single-stranded DNA molecules able to catalyze chemical reactions. There are two main reasons for studying deoxyribozymes: their practical value in various applications, ... -
Nucleoporin-binding nanobodies that either track or trap uclear pore complex assembly
(2021-03-25)Nuclear pore complexes (NPCs) are large multi-protein assemblies that control the transport of macromolecules between the nucleus and the cytoplasm. Their formation from ~ 1,000 individual polypeptides is an impressive ... -
Kinetic Analysis of Mammalian Translation Initiation
(2021-01-22)During translation initiation in mammalian cells, 80S ribosomes are assembled at the translation start site on mRNA via a cap-dependent scanning mechanism. The formation of the 80S initiation complex (IC) is essential for ... -
Investigation of early assembly of OXPHOS complexes during mitochondrial translation
(2020-12-02)After a long time of coevolution between mitochondria and their eukaryotic host cells, the mitochondrial genome has diverged severely from it’s α- proteobacterial ancestors. Mitochondrial genes have mainly been transferred to ... -
Identification of peptide-RNA heteroconjugates by mass spectrometry
(2020-10-12)Proteins and nucleic acids are two of the major constituents of life and their interplay is at the center of most biological processes. A deep understanding of the interaction between these biomolecules is crucial for ... -
A possible functional link between RNA degradation and transcription in Bacillus subtilis
(2020-09-24)Cellular levels of RNA depend on the rate of its synthesis and degradation. While synthesis is performed by RNA polymerase conserved in all domains of life, the enzymes responsible for RNA degradation are more unique ...