Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Cramer, Patrick Prof. Dr."
Now showing items 1-18 of 18
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Structural and functional studies of RNA polymerase II passage through nucleosomes
(2024-03-05)Transcription by RNA polymerase II (Pol II) is an essential process of life occurring in the context of the compact eukaryotic nucleus. While transcription elongation has been extensively studied on linear DNA in vitro, ... -
Multi-omics studies of transcription regulatory mechanisms during human cell fate transition
(2024-02-16)RNA polymerase II (Pol II) transcription is an essential process in living organisms that requires accurate regulation and coordination in order to produce protein-coding and many types of non-coding transcripts. One of ... -
The role of proteasome generated spliced peptides in the adaptive immune response
(2022-05-20)CD8+ T-cells are the crucial component of the adaptive immune system as they survey the cells of the body for the signs of pathogens or tumours and eliminate them when necessary. To do this, T-cells recognise short antigenic ... -
Chromatin reorganization, signaling responses and non-coding RNA regulators in homeostasis and senescence
(2022-04-21)In mammalian cells, a relatively limited fraction of genetic information is utilized in a variety of ways to give rise to distinct cellular phenotypes. Thus, the genotype essentially acts as input for multilayered regulatory ... -
Bioinformatics analysis of multi-omics data elucidates the primary function of Oct4 in gene regulation and pluripotency maintenance
(2022-03-14)The transcription factor (TF) Oct4 is essential for maintaining pluripotency in vitro as well as in vivo. The crucial function of Oct4 in control of pluripotency has been extensively studied during reprogramming of somatic ... -
Bioinformatics analysis of multi-omics data elucidates U2 snRNP function in transcription
(2021-08-16)Transcription by RNA polymerase II (Pol II) is an important step in cell function and regulation. Pol II transcription has been shown to be coupled to pre-mRNA splicing, but the underlying mechanisms remain poorly ... -
Investigating phase separation mechanisms for transcriptional control
(2020-11-13)Transcription of protein-coding genes by RNA polymerase (Pol) II is a highly coordinated process. In metazoan cells, transcription is regulated both at the initiation step by recruitment of the Pol II machinery as well as ... -
Insights into mitochondrial presequence and carrier import pathways
(2020-10-20)The mitochondria of yeast Saccharomyces cerevisiae contains more than 1000 proteins, majority of which are imported from the cytosol. The TIM23 and the TIM22 complexes in the inner mitochondrial membrane are essential ... -
The Mediator kinase module: structural and functional studies in transcription regulation
(2020-10-15)Transcription of protein-coding genes occurs when RNA polymerase II (RNAPII) and general transcription factors (GTFs) assemble at genomic promoter regions, forming a transcription pre-initiation complex (PIC), which ... -
A possible functional link between RNA degradation and transcription in Bacillus subtilis
(2020-09-24)Cellular levels of RNA depend on the rate of its synthesis and degradation. While synthesis is performed by RNA polymerase conserved in all domains of life, the enzymes responsible for RNA degradation are more unique ... -
Monitoring dynamics of protein nascent chain on the ribosome using PET-FCS
(2020-06-24)The ribosome synthesizes proteins according to the sequence of the messenger RNA (mRNA) by progressively adding amino acids to the C-terminus of the nascent peptide. For a protein to become fully functional, it has to fold ... -
Investigation of the effects of the splicing inhibitor Pladienolide B and cancer-related mutations in the SF3b1 protein on pre-mRNA splicing in vitro
(2020-06-11)Pre-mRNA splicing is catalyzed by the spliceosome. A highly dynamic, macromolecular RNP complex is formed out of the U1, U2, U4, U5 and U6 snRNPs and multiple snRNP proteins. During the early stages of spliceosome assembly, ... -
Structure of mammalian RNA polymerase II elongation complex bound by α-amanitin and study of mammalian transcription termination and 3’ end processing
(2019-12-04)Chapter 1 RNA polymerase II (Pol II) is the central enzyme that transcribes eukaryotic protein-coding genes to produce mRNA. The mushroom toxin α-amanitin binds Pol II and inhibits transcription at the step of RNA chain ... -
Multi-omics analysis of transcription kinetics in human cells
(2019-08-01)Transcription of the eukaryotic genome is a highly regulated process which is accomplished in a number of steps, known as the transcription cycle. One of these steps is promoter-proximal pausing, a regulatory halt of the ... -
Structural and Functional Investigation of Promoter Distortion and Opening in the RNA Polymerase II Cleft
(2019-02-11)Transcription of genes is a central process of life that defines how genetic information is used. This determines the metabolic state, morphology and fate of a cell, tissue, organ and the entire organism. In eukaryotes, ... -
Network Dynamics as an Inverse Problem
(2016-12-16)In this thesis, we take a general view on the study of networks from inverse perspectives. By proposing a general representation for the dynamics of networks in terms of explicit dependencies among units, we develop new ... -
Structural and functional investigation of cargo recognition by exportins
(2016-11-02)Nucleocytoplasmic exchange is essential for eukaryotic cells. It proceeds through nuclear pore complexes and is largely mediated by Importin β-like nuclear transport receptors (Impβ-like NTRs). According to the direction ... -
Investigation of prokaryotic immune defense system with quantitative and structural mass spectrometry
(2016-02-26)Bacteria and archaea have evolved an adaptive and heritable immune defense system comprising a CRISPR (clustered regularly interspaced short palindromic repeats) locus and Cas (CRISPR-associated) proteins that targets ...