Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Krebber, Heike Prof. Dr."
Now showing items 1-17 of 17
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Analysis of multiprotein-complex components pulled-down with STRIPAK in Sordaria macrospora
(2022-04-14)The striatin-interacting phosphatase and kinase (STRIPAK)-complex is highly conserved and can be found in fungi and animals. In the filamentous ascomycete Sordaria macrospora (Sm), the SmSTRIPAK plays an important role in ... -
Nuclear mRNA quality control factors Gbp2 and Hrb1 take part in cytoplasmic mRNA surveillance through nonsense-mediated decay
(2022-01-27)Yeast SR-like proteins Gbp2 and Hrb1 shuttle between the nucleus and the cytoplasm, and serve as quality control factors for pre-mRNA splicing. They are loaded onto the RNA co-transcriptionally, and upon correct splicing, ... -
Characterization of the stepwise maturation of the telomerase RNA TLC1 and assembly of the telomerase in Saccharomyces cerevisiae
(2021-11-04)Telomere length homeostasis is a prerequisite for cell viability. It is challenged through successive shortening, which is due to the “end-replication-problem”. This limits the cellular life span in multicellular organisms ... -
Insights into the biogenesis of the human mitochondrial ribosomal large subunit – Characterisation of mL44 and mL45
(2020-11-16)Due to the presence of high-resolution cryo-EM structures of the human mitoribosome, the understanding about the function and assembly of the intriguing mitochondrial ribonucleoprotein complex increased tremendously during ... -
A nuclear export sequence in Nup214 promotes its targeting to the nuclear pore complex
(2020-10-29)The nuclear pore complex (NPC) controls the communication between the nucleus and the cytoplasm in a selective manner via a hydrophobic barrier involving phenylalanine-glycine (FG) repeats. Interactions with components of ... -
Recoding of bacteriophage T4 gene 60 mRNA by programmed translational bypassing
(2020-07-28)During synthesis of a topoisomerase subunit encoded by the gene 60 of bacteriophage T4, the ribosome takes off from a certain codon, slides over a 50-nt non-coding segment of mRNA and resumes translation on a specific ... -
Gbp2 and Hrb1 continue their mRNA quality control in the cytoplasm and take part in Nonsense Mediated Decay
(2019-09-02)The eukaryotic mRNA life cycle is orchestrated by many pathways. Every aspect of this life cycle is associated with quality control factors. This tight coupling of mRNA transcription, processing, export and translation ... -
Analysis of Protein Transport to the Inner Nuclear Membrane
(2019-07-10)The eukaryotic cell is divided into different compartments. The biggest compartment is the nucleus which is formed by one lipid bilayer folded into two membranes: the outer nuclear membrane (ONM) and the inner nuclear ... -
Smu1 and RED play an important role for the activation of human spliceosomes
(2019-07-08)In eukaryotes, pre-mRNA splicing is catalysed by the spliceosome, a highly complex and dynamic molecular machine, which assembles stepwise by the sequential recruitment of five small nuclear ribonucleoproteins (snRNPs) and ... -
Characterization of Npl3-mediated RNA quality control in Saccharomyces cerevisiae
(2018-12-19)During all steps of nuclear RNA maturation, aberrant RNAs can be generated. However, nuclear quality control mechanisms ensure that immature or aberrant RNAs are retained in the nucleus and subsequently degraded to prevent ... -
Identification and Characterization of Importin 13 Substrates
(2018-05-09)Nuclear pore complexes embedded in the nuclear envelope regulate the bidirectional transport of macromolecules between the nucleus and the cytoplasm. Small molecules can rapidly move through the permeability barrier of the ... -
Characterization of mRNA export and nuclear quality control under heat stress in the yeast Saccharomyces cerevisiae
(2017-05-17)Elevated temperatures and other changes of the environment a cell lives in, can have tremendous impact on cellular processes and integrity. To counteract these threats, cells have established various mechanisms to avoid ... -
Monitoring the late events of translation initiation in real-time
(2016-10-19)In bacteria, translation initiation is promoted by three initiation factors (IF1, IF2 and IF3) and entails three distinct steps. First, the small ribosomal subunit (30S) recruits the initiation factors, initiator tRNA ... -
Dynamic changes in cytochrome c oxidase assembly and organization
(2016-08-24)The respiratory chain in the inner membrane of the yeast mitochondrion is organized as a network of individual complexes and large supercomplex structures. These supercomplexes are composed of dimeric complex III and one ... -
Characterization of Rho GTPase GAP/GEF modules in the ascomycete Neurospora crassa
(2015-12-22)Rho (Ras homologue) GTPases are members of the Ras superfamily and known as key players in highly conserved signaling pathways regulating cellular processes like metabolism, survival, differentiation, vesicle transport and ... -
Cross-talk between α-synuclein post-translational modifications in yeast as model of Parkinson’s disease
(2014-10-30)Post-translational modifications modulate several characteristics of proteins. They can regulate substrate activity, localization, stability, but also feature and structure, remarkably in disease-associated proteins. The ... -
Role of methyltransferases in fungal development and secondary metabolite production
(2014-02-17)Fungal development and secondary metabolism are controlled by environmental signals through regulatory proteins. VeA protein is the founding member of the velvet superfamily of fungal regulators. It is involved in light ...