Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Stark, Holger Prof. Dr."
Now showing items 1-20 of 47
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Biochemical Characterization of Otoferlin, the Ca2+ sensor for exocytosis at inner hair cell synapses
(2025-03-27)Transmission of sound from the ear to the brain requires high temporal and spatial fidelity. Inner hair cells (IHC) of the inner ear are the primary sensory organ of hearing, forming the first synapse of the auditory ... -
Mechanisms of Ribosomal Translation studied by Molecular Dynamics Simulations
(2024-12-17)During translation, the ribosome moves along the mRNA and decodes the genetic information to synthesize proteins. This process proceeds in a step-wise iterative manner during which the ribosome undergoes conformational ... -
Release of a Natural mRNA During Recycling of the Prokaryotic Ribosome
(2023-06-20)Ribosome recycling is the last step of the bacterial translation cycle. The release of the peptide leaves a 70S ribosome with a deacylated tRNA in the P site and the stop codon of the mRNA in the A site. The combined action ... -
Structural interrogation of enzyme mechanism and dynamics
(2023-05-05)The pyruvate dehydrogenase complex (PDHc) is one of the largest macromolecular enzyme complexes, and serves an universally important metabolic function by converting pyruvate into acetyl-CoA. Therefore, the PDHc connects ... -
Exploring the human Hsp70-Hop-Hsp90 system for Tau recognition
(2022-03-22)Molecular chaperones are diligent helpers of the cell that ensure a well-balanced proteome by regulating protein production, protein folding, refolding and holding as well as protein degradation. In doing so, molecular ... -
Structural Insights into Early Spliceosome Assembly by cryo-EM
(2021-12-23)In eukaryotes, splicing is a process during which the non-coding introns of the pre-messenger RNA (pre-mRNA) are removed, and the protein-coding exons are ligated. The introns are defined by conserved 5' splice site (5'SS), ... -
Structure-function analysis of human nucleotide excision DNA repair
(2021-04-09)An average human cell accumulates tens of thousands of DNA lesions per day. Therefore, DNA repair pathways are set up to continuously proofread the DNA and correct DNA lesions, thus ensuring accurate expression and ... -
Kinetic Analysis of Mammalian Translation Initiation
(2021-01-22)During translation initiation in mammalian cells, 80S ribosomes are assembled at the translation start site on mRNA via a cap-dependent scanning mechanism. The formation of the 80S initiation complex (IC) is essential for ... -
Advances in enhanced multi-plane 3D imaging and image scanning microscopy
(2021-01-07)High-speed 3D optical microscopy is an indispensable requirement in studying rapid processes like signaling in neuronal networks, flagellar motion, or complex motion of living and highly dynamic subcellular components. ... -
Investigation of early assembly of OXPHOS complexes during mitochondrial translation
(2020-12-02)After a long time of coevolution between mitochondria and their eukaryotic host cells, the mitochondrial genome has diverged severely from it’s α- proteobacterial ancestors. Mitochondrial genes have mainly been transferred to ... -
Reconstitution of Doa10-mediated ER-associated protein degradation with purified components
(2020-11-09)ER-associated protein degradation (ERAD) is a protein quality control pathway at the ER membrane mediated by membrane-embedded ubiquitin ligase complexes. During ERAD, membrane and luminal proteins are ubiquitinated and ... -
Insights into mitochondrial presequence and carrier import pathways
(2020-10-20)The mitochondria of yeast Saccharomyces cerevisiae contains more than 1000 proteins, majority of which are imported from the cytosol. The TIM23 and the TIM22 complexes in the inner mitochondrial membrane are essential ... -
The Mediator kinase module: structural and functional studies in transcription regulation
(2020-10-15)Transcription of protein-coding genes occurs when RNA polymerase II (RNAPII) and general transcription factors (GTFs) assemble at genomic promoter regions, forming a transcription pre-initiation complex (PIC), which ... -
Recoding of bacteriophage T4 gene 60 mRNA by programmed translational bypassing
(2020-07-28)During synthesis of a topoisomerase subunit encoded by the gene 60 of bacteriophage T4, the ribosome takes off from a certain codon, slides over a 50-nt non-coding segment of mRNA and resumes translation on a specific ... -
Monitoring dynamics of protein nascent chain on the ribosome using PET-FCS
(2020-06-24)The ribosome synthesizes proteins according to the sequence of the messenger RNA (mRNA) by progressively adding amino acids to the C-terminus of the nascent peptide. For a protein to become fully functional, it has to fold ... -
Biophysical and Crystallographic Characterization of Spliceosomal DExD/H-box ATPases
(2020-04-23)DEAH-box ATPases play a vital role in the activation, catalytic and disassembly steps of the spliceosome. They are key for the orchestration of conformational and compositional rearrangements during the splicing cycle. It ... -
New sample preparation techniques of macromolecular complexes for high resolution structure determination using cryo-EM
(2020-04-07)Fatty acids are one of the most abundant lipids in the cell. Cells use them to build biological membranes, as energy reserves and as signaling molecules. Fatty acids are synthesized by a specialized protein machinery called ... -
Reaction mechanism of hOMPD and CaAAD at atomic resolution
(2019-12-12)Decarboxylation reactions are fundamental processes in the chemistry of the animated world. Thus, the evolution of decarboxylases brought up a stunning array of diverse enzymes utilizing a variety of different mechanisms ... -
The Role of EF-G in Translational Reading Frame Maintenance on the Ribosome
(2019-09-10)Translation of an mRNA by the ribosome is the final step of gene expression. During translation initiation, the ribosome establishes the mRNA reading frame with the help of initiator tRNA binding to the start codon. This ... -
Recoding of viral mRNAs by –1 programmed ribosome frameshifting
(2019-06-11)–1 programmed ribosome frameshifting (–1PRF) is commonly used in many viruses and cellular genes. The current study focuses on the –1PRF in two human viruses, human immunodeficiency virus type 1 (HIV-1) and Semliki Forest ...