Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Stein, Alexander Dr."
Now showing items 1-19 of 19
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Characterization of Ca2+-triggered (de)ubiquitination events in depolarized synaptosomes by mass spectrometry
(2024-08-15)Communication between neurons takes place at synapses, where the transmission of information from the presynaptic neuron to the postsynaptic cell involves the Ca2+-regulated fusion of synaptic vesicles (SVs) with the ... -
Regulation of the Hrd1 Ubiquitin ligase during protein degradation at the endoplasmic reticulum
(2024-05-16)Misfolded or redundant proteins of the endoplasmic reticulum are degraded via ER associated degradation: They are recognized, undergo a retrotranslocation into the cytosol, become ubiquitinated, are extracted from the ... -
Molecular characterization of protein translocation pores
(2024-02-27)Within the intricate realm of intracellular protein transport, individual organelles are reliant upon precise and dedicated protein machineries, commonly referred to as translocon, to facilitate the translocation of proteins ... -
Biochemical reconstitution of human autophagy initiation
(2023-05-04)Autophagy is a tightly regulated process that eukaryotic cells use as a major survival mechanism to reallocate nutrients to essential processes in adverse conditions such as nutrient or energy deprivation. Autophagy is ... -
Cotranslational folding of HemK C-terminal domain
(2023-03-17)The correct folding of proteins is essential for the survival of the cell. Despite the importance of the question, it remains largely unclear how proteins, in particular large multidomain proteins with mixed α-helical and ... -
Co-translational processing and membrane insertion of proteins in bacteria
(2022-11-04)Protein biogenesis entails the synthesis of polypeptides on the ribosome, their co- and post-translational maturation, folding, and, in the case of membrane proteins, insertion into the lipid bilayer. Bacterial proteins ... -
Modifications in AP2 and clathrin-mediated endocytosis as a mechanism of synaptic plasticity
(2022-07-01)AP2 CME fulfills essential functions in embryonic development and cell survival. The AP2 complex and clathrin form AP2 CCV together with sixty other coat proteins. Due to this complex protein network, the regulation of CCV ... -
The role of proteasome generated spliced peptides in the adaptive immune response
(2022-05-20)CD8+ T-cells are the crucial component of the adaptive immune system as they survey the cells of the body for the signs of pathogens or tumours and eliminate them when necessary. To do this, T-cells recognise short antigenic ... -
Structural Insights into Early Spliceosome Assembly by cryo-EM
(2021-12-23)In eukaryotes, splicing is a process during which the non-coding introns of the pre-messenger RNA (pre-mRNA) are removed, and the protein-coding exons are ligated. The introns are defined by conserved 5' splice site (5'SS), ... -
Investigation of early assembly of OXPHOS complexes during mitochondrial translation
(2020-12-02)After a long time of coevolution between mitochondria and their eukaryotic host cells, the mitochondrial genome has diverged severely from it’s α- proteobacterial ancestors. Mitochondrial genes have mainly been transferred to ... -
Insights into mitochondrial presequence and carrier import pathways
(2020-10-20)The mitochondria of yeast Saccharomyces cerevisiae contains more than 1000 proteins, majority of which are imported from the cytosol. The TIM23 and the TIM22 complexes in the inner mitochondrial membrane are essential ... -
Protein Turnover on Plant Lipid Droplets
(2020-04-30)Lipid droplets (LDs) are lipid storage organelles found across all kingdoms of life. In recent years, the perception of the organelle has shifted from inactive lipid storage sites to dynamic organelles central to the ... -
New sample preparation techniques of macromolecular complexes for high resolution structure determination using cryo-EM
(2020-04-07)Fatty acids are one of the most abundant lipids in the cell. Cells use them to build biological membranes, as energy reserves and as signaling molecules. Fatty acids are synthesized by a specialized protein machinery called ... -
The client spectrum of Get3, an evolutionarily conserved chaperone of membrane proteins
(2020-03-12)Tail-anchored proteins constitute a physiologically important class of membrane proteins. Due to their topology, they are targeted post-translationally to membranes. Although Get3 is responsible for the membrane targeting ... -
Mechanisms of regulation of mitochondria-endoplasmic reticulum contact sites
(2019-11-19)In eukaryotic cells, inter-organelle communication is crucial for several cellular functions, as well as for several cell signaling mechanisms. Mitochondria and the endoplasmic reticulum (ER), for example, form tight contact ... -
Functional analyses of RNA helicases in human ribosome biogenesis
(2019-10-30)Eukaryotic ribosomes are essential ribonucleoprotein (RNP) complexes consisting of four ribosomal RNAs (18S, 5S, 5.8S, 28S/25S) and approximately 80 ribosomal proteins, which are required for the process of translating the ... -
Analysis of Protein Transport to the Inner Nuclear Membrane
(2019-07-10)The eukaryotic cell is divided into different compartments. The biggest compartment is the nucleus which is formed by one lipid bilayer folded into two membranes: the outer nuclear membrane (ONM) and the inner nuclear ... -
Molecular investigation of mitochondrial inner membrane morphology
(2019-06-03)The mitochondrial inner membrane (MIM) is highly curved and displays a complex but extremely organised ultrastructure. It is subdivided into two morphologically, topologically, as well as functionally, distinct compartments. ... -
Molecular Characterization of the Mitochondrial Presequence Translocase
(2018-01-31)In this thesis, multiple pore-lining amino acid residues of the mitochondrial presequence translocation channel Tim23 were identified as constituents of the channel’s cation filter by combining single channel electrophysiology ...