Functions of cytoplasmic lattices in mammalian oocytes and embryosDoctoral thesis
Date of Examination:2023-01-19
Date of issue:2023-12-12
Advisor:Prof. Dr. Melina Schuh
Referee:Prof. Dr. Peter Rehling
Referee:Dr. Peter Lenart
Referee:Prof. Dr. Laurinda Jaffe
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EnglishPreimplantation embryogenesis is dependent on maternally stored proteins, transcripts, and other macromolecules. Although this is a well-established dogma, it is unclear how mammalian oocytes store maternal factors needed following fertilization. In this study, we combined transcriptomics, proteomics, super-resolution light microscopy and structural electron tomography to investigate how oocytes accumulate and store maternal factors for early embryogenesis. My work reveals an unexpected mechanism of how the oocyte supports early embryo development. Together, the data presented within this thesis provide new insights into the poorly understood mechanism of maternal storage in mammalian oocytes and links it to the leading genetic causes of human infertility.
Keywords: oocyte; embryo; cytoplasmic lattices; maternal-to-zygotic transition