About 50 years ago, even before NMR was ready to determine structure of proteins, it could unveil a surprising motion in aromatic side-chain (Wuthrich & Wagner, 1975) from 1D 1H spectra. With decades of development, NMR ...

Hyperpolarization methods have become a powerful assistance technique to NMR spectroscopy in order to improve its sensitivity and consequently reduce the required measurement time to achieve a satisfactory signal-to-noise ...

Protein folding and molecular recognition are physiological processes that are intricately linked to their dynamic signature and reflected in their thermal coefficients. Additionally, the local conformational entropy is ...

Stereochemistry is a very important subject in chemistry and life sciences, as most of the biologically active molecules, including proteins, oligonucleotides, sugars, lipids and small molecules exist in one chiral form. ...

Two methods to obtain paramagnetically tagged oligonucleotides are described. To get a biomolecule with a paramagnetic center a radical or metal moiety has to be introduced. This was done ...

This thesis sheds light on the mode of action of membrane proteins and their ligands. Two model systems were chosen: the membrane protein was the human voltage dependent anion channel (HVDAC) ...

The formation of the Tat-protein/TAR RNA complex is a crucial step in the regulation of Human Immunodeficiency Virus (HIV)-gene expression. To obtain fulllength viral transcripts the Tat/TAR ...

The present work provides new structural information about bacterial proteins involved in transcription regulation and protein secretion, as well as a contribution to the improvement of ...