| dc.contributor.advisor | Schwappach-Pignataro, Blanche Prof. Dr. | |
| dc.contributor.author | Farkas, Ákos | |
| dc.date.accessioned | 2020-03-12T10:26:31Z | |
| dc.date.available | 2021-11-18T00:50:07Z | |
| dc.date.issued | 2020-03-12 | |
| dc.identifier.uri | http://hdl.handle.net/21.11130/00-1735-0000-0005-1360-9 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-7919 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-7919 | |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject.ddc | 572 | de |
| dc.title | The client spectrum of Get3, an evolutionarily conserved chaperone of membrane proteins | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Schwappach-Pignataro, Blanche Prof. Dr. | |
| dc.date.examination | 2019-11-20 | |
| dc.description.abstracteng | Tail-anchored proteins constitute a physiologically important class of membrane
proteins. Due to their topology, they are targeted post-translationally to membranes.
Although Get3 is responsible for the membrane targeting of several ER-bound TA
proteins, our knowledge on its in-vivo client spectrum remains limited. The oxidation-induced
chaperone activity of Get3 detected in-vitro suggests that Get3 may handle
further substrates besides TA proteins. My bioinformatic analysis shows that Get3-like
chaperones are more widespread than previously expected and suggests that a general
chaperone activity is the ancestral function of Get3. Using budding yeast as a model
system, I show that although Get3 can target many TA proteins, only a fraction of them
are obligate substrates of Get3. I provide evidence that an essential TA protein, Sed5, is
a super-client of Get3 that interacts with it before and also after membrane targeting. I
also show that Get3 can target proteins with multiple transmembrane segments located
proximal to their C-terminus as part of the GET pathway. Finally, I describe membrane
proteins with amphipathic helices as potential chaperone substrates of Get3. Therefore,
the current study significantly expands the range of potential Get3 clients in vivo and
indicates that Get3 is an important chaperone of membrane proteins both before and after
membrane targeting. | de |
| dc.contributor.coReferee | Stein, Alexander Dr. | |
| dc.subject.eng | Get3 | de |
| dc.subject.eng | tail-anchored | de |
| dc.subject.eng | chaperone | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-21.11130/00-1735-0000-0005-1360-9-7 | |
| dc.affiliation.institute | Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) | de |
| dc.subject.gokfull | Biologie (PPN619462639) | de |
| dc.description.embargoed | 2021-11-18 | |
| dc.identifier.ppn | 1692414275 | |