α-synuclein disrupts neuron network rhythmic activity when overexpressed in cultured neurons

Leite, Kristian

dc.contributor.advisor	Kügler, Sebastian Dr.
dc.contributor.author	Leite, Kristian
dc.date.accessioned	2021-03-09T13:08:01Z
dc.date.available	2022-02-07T00:50:04Z
dc.date.issued	2021-03-09
dc.identifier.uri	http://hdl.handle.net/21.11130/00-1735-0000-0005-159C-4
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-8468
dc.description.abstract	Synuclein, Parkinson's disease, network activity, neuron, tau protein, neurodegeneration, connectivity, cAMP,	de
dc.language.iso	eng	de
dc.relation.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	570	de
dc.title	α-synuclein disrupts neuron network rhythmic activity when overexpressed in cultured neurons	de
dc.type	doctoralThesis	de
dc.contributor.referee	Dresbach, Thomas Prof. Dr.
dc.date.examination	2021-02-09
dc.description.abstracteng	Synucleinopathies are associated with a generalized slowing of the brain’s rhythmic activity, which co-occurs with dementia and has been connected with increased levels of α synuclein in both patients and test animals. In this study, the impact of increased levels of α synuclein on rhythmic activity was analyzed in vitro, with the intention of identifying the mechanisms behind the disruptive role of this protein. It was thus determined that increased levels of α-synuclein reduced the amount of structures involved in neuronal connectivity and reduced the levels of the signaling molecule cAMP. It was further determined that the reduction of burst frequency did not involve extracellular α-synuclein released by synuclein-overexpressing neurons, contradicting the results of previous studies. Finally, our study observed that in cases of tau protein or P123H β-synuclein mutant overexpression, the frequency of rhythmic activity was lowered alongside a simultaneous reduction of both structures involved in neuronal connectivity and levels of cAMP.	de
dc.contributor.coReferee	Zweckstetter, Markus Prof. Dr.
dc.subject.eng	Synuclein	de
dc.subject.eng	Parkinson's disease	de
dc.subject.eng	Tau protein	de
dc.subject.eng	Network bursts	de
dc.subject.eng	Network activity	de
dc.subject.eng	Neurodegeneration	de
dc.subject.eng	Connectivity	de
dc.subject.eng	cAMP	de
dc.subject.eng	P123H	de
dc.subject.eng	Rhythmic activity	de
dc.subject.eng	Automated analysis of neuronal activity	de
dc.subject.eng	ImageJ analysis	de
dc.subject.eng	AAV viruses	de
dc.subject.eng	Calcium imaging	de
dc.subject.eng	Fluorescence microscopy	de
dc.subject.eng	Neuronal cell culture	de
dc.identifier.urn	urn:nbn:de:gbv:7-21.11130/00-1735-0000-0005-159C-4-4
dc.affiliation.institute	Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.description.embargoed	2022-02-07
dc.identifier.ppn	175089890X

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GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften [1031]
GGNB - Göttingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences

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