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Functional Characterisation of Syndecan, a heparan sulpahte proteoglycan, in Slit/Robo signalling

dc.contributor.advisorVorbrüggen, Gerd Dr.de
dc.contributor.authorChanana, Bhavnade
dc.date.accessioned2013-01-22T15:44:49Zde
dc.date.available2013-01-30T23:50:58Zde
dc.date.issued2008-04-25de
dc.identifier.urihttp://hdl.handle.net/11858/00-1735-0000-000D-F150-Fde
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-3446
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-3446
dc.description.abstractSyndecan (Sdc) ist ein Transmembranprotein der Klasse I, das im Bereich seiner extrazellulären Domäne mit mehreren linearen Zuckerketten modifiziert ist. Diese Zuckerketten werden als Heparansulfat-Glykosaminoglykane (HSGAG) bezeichnet. In vorhergehenden Experimenten konnte eine essentielle Funktion des Sdc-Proteins für die Slit-Signaltransduktion an der Mittellinie des Drosophila Embryos gezeigt werden. Das sezernierte Slit-Protein über die Bindung an den Robo-Rezeptor als abstoßende Aktivität, die sowohl die Axone des zentralen Nervensystems (ZNS) als auch die Muskulatur im ventralen Bereich des Embryos strukturiert. Die vorliegende Doktorarbeit hatte das Ziel, ein besseres Verständnis der Funktion des Sdc-Proteins innerhalb des Slit/Robo-Signaltransduktionsweges zu gewinnen. Mit Hilfe des UAS/GAL4-Systems wurden verschiedene Sdc-Proteinvarianten und Proteinde
dc.format.mimetypeapplication/postscriptde
dc.language.isoengde
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/de
dc.titleFunctional Characterisation of Syndecan, a heparan sulpahte proteoglycan, in Slit/Robo signallingde
dc.typedoctoralThesisde
dc.title.translatedFunktionale Charakterisierung von Syndecan, ein Heparansulfatproteoglykan, im Slit/Robo-Signalwegde
dc.contributor.refereeVorbrüggen, Gerd Dr.de
dc.date.examination2007-11-06de
dc.subject.dnb500 Naturwissenschaftende
dc.subject.gokWKF 300de
dc.description.abstractengSyndecan (Sdc) is a type I transmembrane protein characterized by the presence of linear sugar chains called heparan sulphate glycosaminoglycans (HSGAGs) linked to its extracellular domain. Sdc was shown to be critical for the fidelity of Slit repellent signalling at the Drosophila midline where via its receptor Robo, Slit patterns the central nervous system (CNS) and the musculature. This study aimed at shedding some light on how Sdc functions in Slit/Robo signalling. Employing the UAS/GAL4 system, different sdc variants and chimeric transgenes were expressed in specific tissues in a sdc mutant background and tested for their rescue capability. Rescue was scored by the absence of ventral midline crossing of ipsilateral axons and ventral muscles. The in vivo rescue experiments proved that Drosophila Sdc is specifically required on the target tissue where it functions as a coreceptor with Robo and that Sdc has no function in Slit secretion or transport nor does it have an independent intracellular signalling activity. Furthermore, the results also support a model in which shedding of Sdc though not required for the fidelity of Slit repellent signalling could occur by the proteolytic activity of a serine protease which might function as a negative feedback system or might be required for the recycling of Robo receptor.de
dc.contributor.coRefereeWimmer, Ernst A. Prof. Dr.de
dc.contributor.thirdRefereePieler, Tomas Prof. Dr.de
dc.subject.topicMathematics and Natural Sciencede
dc.subject.gerSyndecande
dc.subject.gerHeparansulfatproteoglykande
dc.subject.gerSlitde
dc.subject.gerRobode
dc.subject.gerAxonede
dc.subject.engSyndecande
dc.subject.engheparan sulphate proteoglycande
dc.subject.engSlitde
dc.subject.engRobode
dc.subject.engaxon guidancede
dc.subject.bk42.23de
dc.identifier.urnurn:nbn:de:gbv:7-webdoc-1770-5de
dc.identifier.purlwebdoc-1770de
dc.identifier.ppn588951625de


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