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Nucleosylaminosäuren als Bausteine zur Synthese modifizierter Oligonucleotide

dc.contributor.advisorDucho, Christian Prof. Dr.
dc.contributor.authorSchmidtgall, Boris
dc.date.accessioned2014-05-13T09:28:02Z
dc.date.available2014-05-13T09:28:02Z
dc.date.issued2014-05-13
dc.identifier.urihttp://hdl.handle.net/11858/00-1735-0000-0022-5EB1-0
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-4502
dc.language.isodeude
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc571.4de
dc.titleNucleosylaminosäuren als Bausteine zur Synthese modifizierter Oligonucleotidede
dc.typedoctoralThesisde
dc.title.translatedNucleosyl Amino Acids as Building Blocks for the Synthesis of Modified Oligonucleotidesde
dc.contributor.refereeDucho, Christian Prof. Dr.
dc.date.examination2014-05-09
dc.description.abstractengIt has been proposed that biopolymers need to be uniformly charged to form self-sustaining chemical systems. We have therefore established a method for the site-selective replacement of natural phosphodiester linkages of oligonucleotides (ON) by the positively charged NAA (nucleosyl amino acid) motif. The structure of these building blocks was inspired by naturally occurring muraymycin antibiotics. The phosphoramidite reagents were used to prepare various modified DNA-ON by solid phase-supported synthesis. The resultant modified ON were investigated with respect to structural and thermodynamic properties. Melting experiments revealed only a slight decrease in thermal stability of duplices bearing one NAA-modified strand. Further results indicated that mismatch recognition is not harmed and no structural distortions of duplices occur. These results challenge the hypothesis of the uniform charge pattern to be physico-chemically necessary for replicable biopolymers.de
dc.contributor.coRefereeDiederichsen, Ulf Prof. Dr.
dc.subject.gerModifizierte Nucleinsäurende
dc.subject.gerNucleosidede
dc.subject.gerNucleosylaminosäurende
dc.subject.engmodified nucleic acidsde
dc.subject.engnucleosidesde
dc.subject.engnucleosyl amino acidsde
dc.identifier.urnurn:nbn:de:gbv:7-11858/00-1735-0000-0022-5EB1-0-9
dc.affiliation.instituteGöttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)de
dc.subject.gokfullBiologie (PPN619462639)de
dc.identifier.ppn785581766


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