| dc.contributor.advisor | Jahn, Reinhard Prof. Dr. | |
| dc.contributor.author | Jakhanwal, Shrutee | |
| dc.date.accessioned | 2018-02-16T10:03:58Z | |
| dc.date.available | 2018-02-16T10:03:58Z | |
| dc.date.issued | 2018-02-16 | |
| dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-002E-E355-D | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-6731 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-6731 | |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject.ddc | 570 | de |
| dc.title | Regulation of the neuronal SNARE-complex by accessory proteins | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Steinem, Claudia Prof. Dr. | |
| dc.date.examination | 2017-07-13 | |
| dc.description.abstracteng | In this work, I have thoroughly characterized a novel complex containing syntaxin1a (1-288), SNAP25a and Munc18-1 and established it as an efficient acceptor complex for synaptobrevin-binding, using several biochemical and biophysical techniques. The use of all full-length proteins provide tremendous strength to the study, giving it a ‘near-native’ scenario for the behaviors of the proteins being discussed.
In addition to understanding the functional importance of this complex, I also attained its structural overview using chemical crosslinking assays. And, finally, by testing the resistance of this complex to disassembly by NSF-αSNAP, I could establish that the syntaxin1a/SNAP25a/Munc18-1 complex can allow SNARE-assembly to proceed in an NSF-αSNAP-resistant manner. | de |
| dc.contributor.coReferee | Rizzoli, Silvio Prof. Dr. | |
| dc.subject.eng | SNAREs, Munc18, synaptic vesicle exocytosis | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-002E-E355-D-4 | |
| dc.affiliation.institute | Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) | de |
| dc.subject.gokfull | Biologie (PPN619462639) | de |
| dc.identifier.ppn | 1014290279 | |