Blättern GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften nach "Rodnina, Marina Prof. Dr." Betreuer
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Regulation of human 48S initiation complex formation
(2024-04-23)During the initiation phase of protein synthesis in humans, the small ribosome subunit interacts with initiator tRNA (tRNAi) and at least ten different initiation factors (eIFs) to form the initiation complex. The initiation ... -
Elongation factor P: mechanism of action and opportunities for drug design
(2023-07-24)EF-P is a conserved protein that has shape similarity to the tRNAs and binds to the E site of the ribosome. EF-P enhances the rate of peptide bond formation during the synthesis of polyPro sequences. In E. coli one third ... -
Release of a Natural mRNA During Recycling of the Prokaryotic Ribosome
(2023-06-20)Ribosome recycling is the last step of the bacterial translation cycle. The release of the peptide leaves a 70S ribosome with a deacylated tRNA in the P site and the stop codon of the mRNA in the A site. The combined action ... -
Cotranslational folding of HemK C-terminal domain
(2023-03-17)The correct folding of proteins is essential for the survival of the cell. Despite the importance of the question, it remains largely unclear how proteins, in particular large multidomain proteins with mixed α-helical and ... -
Co-translational processing and membrane insertion of proteins in bacteria
(2022-11-04)Protein biogenesis entails the synthesis of polypeptides on the ribosome, their co- and post-translational maturation, folding, and, in the case of membrane proteins, insertion into the lipid bilayer. Bacterial proteins ... -
Ribosome dynamics during spontaneous frameshifting
(2022-10-18)During protein synthesis, the ribosome moves along the mRNA in steps of three nucleotides at a time to maintain the translational reading frame. However, on some mRNA sequences that are denoted as slippery, ribosomes may ... -
Kinetic Analysis of Mammalian Translation Initiation
(2021-01-22)During translation initiation in mammalian cells, 80S ribosomes are assembled at the translation start site on mRNA via a cap-dependent scanning mechanism. The formation of the 80S initiation complex (IC) is essential for ... -
Recoding of bacteriophage T4 gene 60 mRNA by programmed translational bypassing
(2020-07-28)During synthesis of a topoisomerase subunit encoded by the gene 60 of bacteriophage T4, the ribosome takes off from a certain codon, slides over a 50-nt non-coding segment of mRNA and resumes translation on a specific ... -
Mechanism of Ribosome Rescue by Alternative Release Factor B
(2020-07-09)Rescue of ribosomes stalled on non-stop mRNAs is essential in bacteria and in the mitochondria of all eukaryotes. The lack of a stop codon causes ribosomes to stall at the 3’ end of mRNA with an empty A site, and specialized ... -
Monitoring dynamics of protein nascent chain on the ribosome using PET-FCS
(2020-06-24)The ribosome synthesizes proteins according to the sequence of the messenger RNA (mRNA) by progressively adding amino acids to the C-terminus of the nascent peptide. For a protein to become fully functional, it has to fold ... -
Programmed Translational Readthrough in Drosophila melanogaster
(2020-06-05)Translational readthrough (TR) is extensively used by viruses to expand their limited genome capacity. The pervasiveness of TR across eukaryotic genomes is only recently being explored. Systems biology approaches such as ... -
The Role of EF-G in Translational Reading Frame Maintenance on the Ribosome
(2019-09-10)Translation of an mRNA by the ribosome is the final step of gene expression. During translation initiation, the ribosome establishes the mRNA reading frame with the help of initiator tRNA binding to the start codon. This ... -
Recoding of viral mRNAs by –1 programmed ribosome frameshifting
(2019-06-11)–1 programmed ribosome frameshifting (–1PRF) is commonly used in many viruses and cellular genes. The current study focuses on the –1PRF in two human viruses, human immunodeficiency virus type 1 (HIV-1) and Semliki Forest ... -
Analysis of translational fidelity in cellular proteins
(2019-03-27)The accuracy of protein biosynthesis determines the quality of the proteome and regulates the fitness of the cell. A comprehensive quantification of miscoding events in the cell is essential to understand the basic mechanisms ... -
Recognition Elements for Elongation Factor P on the Ribosome
(2019-01-25)Translation elongation is a multi-step process orchestrated by elongation factors. Elongation factors G and Tu are required for each round of translation elongation, whereas elongation factor P is only required to assist ... -
Kinetic models of sequential initiation events upon polysome formation
(2017-09-08)Polysomes are active protein-synthesizing units consisting of multiple ribosomes assembled along one mRNA. The frequency of ribosome loading on the mRNA defines the number of ribosomes per polysome, which is an important ... -
Kinetics of subunit rotation of the ribosome during tRNA-mRNA translocation
(2017-07-25)Ribosome dynamics play an essential role in orchestrating all stages of protein synthesis. Recent biochemical, structural and computational studies have shown large-scale conformational changes of the ribosome, its tRNA ... -
Ribosome Processivity and Co-translational Protein Folding
(2017-04-21)Synthesis of polypeptide chains on the ribosome occurs at a non-uniform rate. mRNA sequences translated at a high rate are interspersed by segments that lead to translational pausing. The rate of translation elongation is ... -
Monitoring the late events of translation initiation in real-time
(2016-10-19)In bacteria, translation initiation is promoted by three initiation factors (IF1, IF2 and IF3) and entails three distinct steps. First, the small ribosomal subunit (30S) recruits the initiation factors, initiator tRNA ... -
Mechanisms of programmed ribosomal -1 frameshifting in bacteria
(2014-07-15)Translation of proteins must be accurate to synthesize functional proteins. However, in some cases gene information is reprogrammed which overwrites the normal decoding rules of translation. One of these cases is programmed ...