Blättern GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften nach "Stark, Holger Prof. Dr." Betreuer
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Structural interrogation of enzyme mechanism and dynamics
(2023-05-05)The pyruvate dehydrogenase complex (PDHc) is one of the largest macromolecular enzyme complexes, and serves an universally important metabolic function by converting pyruvate into acetyl-CoA. Therefore, the PDHc connects ... -
Purification of organellar macromolecular complexes for structural biology
(2022-03-14)Photosynthesis is the source of life on Earth. In oxygenic photosynthesis, water and carbon dioxide are converted into organic matter using of solar energy that results in the release of oxygen. PSII, a supra-molecular ... -
Structural Insights into Early Spliceosome Assembly by cryo-EM
(2021-12-23)In eukaryotes, splicing is a process during which the non-coding introns of the pre-messenger RNA (pre-mRNA) are removed, and the protein-coding exons are ligated. The introns are defined by conserved 5' splice site (5'SS), ... -
New sample preparation techniques of macromolecular complexes for high resolution structure determination using cryo-EM
(2020-04-07)Fatty acids are one of the most abundant lipids in the cell. Cells use them to build biological membranes, as energy reserves and as signaling molecules. Fatty acids are synthesized by a specialized protein machinery called ... -
Structural Characterization of the Eukaryotic Translation Initiation by Electron Cryo-Microscopy
(2019-05-08)Translation is a highly conserved molecular process during which the genetic information stored in an mRNA molecule is translated into a polypeptide chain that eventually folds into functional three-dimensional proteins. ... -
Structure-based mechanistic analysis of the proteasome
(2019-05-02)The ubiquitin proteasome pathway is one of the major protein degradation pathways in the cell. This pathway is essential for protein quality control and the regulation of the cell cycle. Proteins subjected to the ubiquitin ... -
Computational methods for the structure determination of highly dynamic molecular machines by cryo-EM
(2019-02-20)In the last couple of years, electron cryomicroscopy (cryo-EM) has gained of rising importance in the field of structural biology and biophysics. Not only that the routinely achievable resolution of the method has ... -
High-resolution structure determination of human spliceosome complexes by cryo-EM
(2019-02-14)The eukaryotic splicing of precursors to mRNA is facilitated by a highly dynamic, multi-megadalton macromolecular machine termed the spliceosome. The underlying chemical reaction features the excision of an intron, which ... -
New Computational Tools for Sample Purification and Early-Stage Data Processing in High-Resolution Cryo-Electron Microscopy
(2019-01-07)Large macromolecular protein complexes play an important role in the quest of un- derstanding life at a molecular level. For this endeavor, the highly-resolved 3D structure of a large complex and its changes, which are ... -
Structural Characterization of Proteasome Inhibition
(2017-08-15)The proteasome plays a major role in non-lysosomal protein degradation pathways and inhibition of proteasomes became a valid therapeutic strategy for anti-cancer therapy in the recent years. Current efforts focus on the ... -
Structural Survey on Cohesin and Viomycin Inhibited 70S Ribosome by Single Particle Electron Microscopy
(2016-04-21)Life can be understood as the systematic collaborative action of several entities on molecular level reducing locally the entropy at the cost of the exterior environment. These entities are in great part macromolecular ... -
Conformational Dynamics of large protein Complexes
(2015-02-18)Life on earth is only possible through the enormous capabilities of proteins and their assemblies. Which function a certain protein fulfills is encoded in its amino acid sequence, which gives rise to a defined structure. ... -
Strategies to stabilize RNP complexes for structural determination by 3D cryo-electron microscopy
(2014-10-24)The physiological reactions in a cell are generally not performed by single biological macromolecules, but by complexes of several molecules. They can be a complex of several proteins or can be composed of RNA and proteins ... -
Methods to improve the sample quality of macromolecular complexes for structure determination by 3D Electron Cryo-Microscopy
(2012-05-15)Macromolecular assemblies often undergo substantial structural rearrangements. Not only do functional states differ from each other, a single functional state also exhibits inherent conformational heterogeneity. A typical ... -
Three-dimensional electron microscopy of structurally heterogeneous biological macromolecules
(2009-10-28)Biological macromolecules exert their biological functions in a dynamic network of large assemblies. The biological function of these macromolecular assemblies is closely related to dynamic ... -
New Algorithms for Macromolecular Structure Determination
(2009-10-28)Detailed three dimensional information of macromolecules is often crucial to the study of biological systems. Structural data have for example been used to elucidate the basis of diseases ... -
NEW COMPUTATIONAL METHODS FOR 3D STRUCTURE DETERMINATION OF MACROMOLECULAR COMPLEXES BY SINGLE PARTICLE CRYO-ELECTRON MICROSCOPY
(2009-04-22)In cryo-electron microscopy (cryo-EM) single-particle reconstruction, projection images of many first order structural identical copies of the specimen of interest are combined to recover ...