Dokumente GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften nach Gutachter "Ficner, Ralf Prof. Dr."
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A protein in search of a function: The c-di-AMP-binding protein DarA of Bacillus subtilis
(2019-07-26)Adaptation to changing environmental conditions is crucial for any organism to thrive in nature. Bacteria like the Gram-positive model organism Bacillus subtilis have evolved so-called second messengers to facilitate ... -
Absolute and relative quantification of proteins in large protein-RNA assemblies by mass spectrometry
(2010-09-07)The majority of cellular processes is driven by protein complexes. These emerge as multimeric protein assemblies or are complexed with ligands such as RNA or DNA. To understand the details ... -
Biophysical and Crystallographic Characterization of Spliceosomal DExD/H-box ATPases
(2020-04-23)DEAH-box ATPases play a vital role in the activation, catalytic and disassembly steps of the spliceosome. They are key for the orchestration of conformational and compositional rearrangements during the splicing cycle. It ... -
Characterization of Acyltransferases in Neutral Lipid Synthesis
(2021-04-23)Wax ester (WE) are neutral lipids, which consist of a fatty acid and a fatty alcohol moiety connected by an ester bond. WE are synthesized via two reactions from acyl-coenzyme A (CoA)/acyl carrier protein (ACP) substrates. ... -
Characterization of mRNA export and nuclear quality control under heat stress in the yeast Saccharomyces cerevisiae
(2017-05-17)Elevated temperatures and other changes of the environment a cell lives in, can have tremendous impact on cellular processes and integrity. To counteract these threats, cells have established various mechanisms to avoid ... -
Collective Dynamics Underlying Allosteric Transitions: A Molecular Dynamics Study
(2013-01-15)In all living cells, proteins are performing a vast amount of functions. These functions are often controlled by a mechanism called allosteric regulation. In allosteric regulation, binding affinities in one site are ... -
Dynamic interactions during ribosome targeting to the membrane
(2015-05-11)The signal recognition particle (SRP) and its receptor are the main components of the co-translational protein targeting pathway by which membrane proteins are targeted to the membrane. The co-translational targeting pathway ... -
Dynamics and Driving Forces of Macromolecular Complexes
(2013-06-05)Many functions in living cells are governed by macromolecular complexes. To fully describe the underlying mechanisms, they have to be understood at atomic level. The present study combines data obtained by X-ray crystallography ... -
Functional analyses of RNA helicases in human ribosome biogenesis
(2019-10-30)Eukaryotic ribosomes are essential ribonucleoprotein (RNP) complexes consisting of four ribosomal RNAs (18S, 5S, 5.8S, 28S/25S) and approximately 80 ribosomal proteins, which are required for the process of translating the ... -
Functional and Structural Characterization of the DEAH-box Helicase Prp43
(2022-11-24)RNA helicases of the DEAH-box family fulfill essential functions in various aspects of RNA metabolism by reorganizing structured RNAs and RNPs through ATP-dependent motility in 3’ to 5’ direction along single-strands. Prp43 ... -
Functional characterisation of RNA helicases in the remodelling of pre-ribosomal subunits
(2018-07-13)DEAD/H-box RNA helicases are ubiquitously expressed enzymes, which are characterised by a conserved helicase core of two RecA-like domains containing sequence motifs required for substrate binding and hydrolysis of NTP. ... -
Identification of novel components involved in selective and unselective autophagic pathways
(2012-04-11)Autophagy is a ubiquitous protein degradation pathway in eukaryotic cells. As response upon nutrient starvation, cytosolic material and also whole organelles are engulfed by a double membrane-layered vesicle, the ... -
Identification of nuclear export signals and structural analysis of transport complexes.
(2013-01-09)The nucleus is a vital sub cellular compartment and it stores the genome. Transport across the nuclear envelope occurs through Nuclear Pore Complexes (NPC) and cargo traffic across the NPCs is a highly specific and ... -
Influence of Ions on the Assembly of Vimentin Intermediate Filaments
(2020-07-10)The human body consists of many different cell types, which all exhibit a cytoskeleton. This cytoskeleton is mainly composed of three different types of protein filaments, as well as cross-linkers and motor proteins. The ... -
Insertion of an intrinsically disordered domain in VelB supports selective heterodimer formation of fungal velvet domain regulatory proteins in Aspergillus nidulans
(2019-04-10)The fungal specific velvet domain mediates DNA-binding and protein-protein interaction and is structurally similar to the Rel homology domain of the mammalian immune and infection response NF-ᴋB regulator. Homo- and ... -
Insights into the activation of the spliceosomal helicase Prp43
(2013-11-20)The excision of non-coding sequences that are present in the pre-mRNA is a hallmark of eukaryotic cells. This process is termed as splicing and is catalysed by the spliceosome, a single-turnover enzyme which consists of ... -
Interactions and functions of RNA-binding proteins
(2018-04-13)Alongside several well-known modifications in DNA and proteins, more than 100 different types of chemical modification are also found in cellular RNAs. RNA modifications can influence the secondary structure and interactions ... -
Investigation of DNA in Cell Nuclei by Combined Imaging Techniques
(2021-03-31)Biological cells are the basic structural and functional units of all organisms. There are approximately 60 trillion eukaryotic cells within a human body alone. A common feature among eukaryotic cells is the nucleus, a ... -
Investigation of Nucleosome Dynamics by Genetic Code Expansion
(2015-10-28)The DNA of eukarytic cells is compacted into a complex macromolecular structure called chromatin. Histones are the small structural proteins that form central octamers around which the DNA is wrapped to form the basic ... -
Investigation of the 3D structure of the human activated spliceosome by cryo-electron microscopy
(2018-04-04)The spliceosome is a highly dynamic megadalton ribonucleoprotein (RNP) complex that catalyses the removal of introns from eukaryotic precursor messenger RNA (pre mRNA) in two consecutive transesterification reactions. The ...