Dokumente GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften nach Gutachter "Grubmüller, Helmut Prof. Dr."
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Bayesian Structural Ensemble Determination from Single-Molecule X-ray Scattering
(2024-02-09)Single-molecule X-ray scattering experiments using ultrashort X-ray free electron laser (XFEL) pulses have opened a new route for the structure determination of biomolecules. They also hold the potential to extract the ... -
Droplet-based giant unilamellar vesicles as a tool for pore-spanning membrane preparation and transmembrane protein reconstitution
(2022-11-22)Model membranes represent a central tool to decipher complex processes of living cells. Giant unilamellar vesicles (GUVs) are of particular interest as they are cell-like in size, observable with optical microscopy and ... -
Spatially Resolved Hydration Statistical Mechanics at Biomolecular Surfaces from Atomistic Simulations
(2021-03-18)The statistical mechanics of the first few hydration layers is vital for many biophysical processes, such as protein folding and unfolding, protein function, lipid bilayer self-assembly, and ligand binding. These processes ... -
On the Use of Temporal Information for the Reconstruction of Magnetic Resonance Image Series
(2020-10-28)Standard clinical magnetic resonance imaging uses data sampled at the Nyquist rate which limits it to frame rates of 1-4 frames per second. Over the past decade different methods for the reconstruction of images from ... -
Studying Molecular Interactions under Flow with Fluorescence Fluctuation Spectroscopy
(2020-06-11)Assembly and interactions of biomolecules are fundamental processes in living organisms to maintain the physiological cellular behavior. The majority of the time protein assembly is useful and vital for the organism. ... -
Development and characterization of novel reversibly switchable red fluorescent proteins with opposing switching modes
(2020-03-03)Since the discovery of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria, a multitude of fluorescent proteins have been derived from the original GFP and its homologs by protein engineering. Their ... -
Extending Resolution in All Directions: Image Scanning Microscopy and Metal-induced Energy Transfer
(2019-03-18)Fluorescence microscopy is a powerful tool in the life sciences and is used to study structure and function on length scales from cells down to single molecules. In recent years, fluorescence microscopy has seen enormous ... -
Computational methods for the structure determination of highly dynamic molecular machines by cryo-EM
(2019-02-20)In the last couple of years, electron cryomicroscopy (cryo-EM) has gained of rising importance in the field of structural biology and biophysics. Not only that the routinely achievable resolution of the method has ... -
Stochastic modeling of photoswitchable fluorophores for quantitative superresolution microscopy
(2017-04-13)The diffraction limit of optical microscopy can be overcome by switching fluorophores between on- and off-states. In the on-state, the fluorophores emit fluorescence and can be detected. In the off-state, they remain dark. ... -
Conformational Changes in Ligand Binding Processes
(2015-03-11)Ligand and protein-protein binding processes play a crucial role in many biological processes. In many cases ligand binding is associated with conformational changes within the receptor protein. While structure determination ... -
Fluorescence spectroscopic studies of protein conformational dynamics
(2013-10-31)The folding of a protein into its native structure is the fundamental prerequisite for its functionality. The polypeptide chain itself contains the chemical information necessary for spontaneous folding of the protein. ... -
Dynamics and Driving Forces of Macromolecular Complexes
(2013-06-05)Many functions in living cells are governed by macromolecular complexes. To fully describe the underlying mechanisms, they have to be understood at atomic level. The present study combines data obtained by X-ray crystallography ... -
Diffusion and Flow on Microscopic Length Scales Studied with Fluorescence Correlation Spectroscopy
(2012-10-30)The physics of fluids on microscopic lengthscales is fundamentally different from the one governing macroscopic fluid phenomena. On lengthscales up to a few micrometer, inertial forces of small particles and fluid elements are ... -
Protonation patterns in reduced and oxidized form of electron transfer proteins
(2012-10-23)The pKa's of the ionizable amino acids are crucial for the function of many proteins as they are key factors that determine their electrostatic potential and its spatial distribution, often controlling and optimizing ... -
STED nanoscopy of the living brain
(2012-05-03)Over the past decade, a new class of fluorescence microscopes has evolved which thoroughly break the diffraction limit of classical far-field light microscopy and thus provide significantly improved spatial resolution. ...