Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Jakobs, Stefan Prof. Dr."
Now showing items 1-20 of 21
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Advancements in Nanobody Applications: From Live Synaptic Imaging to Multiplexed Fluorescence Microscopy and Nanoscale Topography Mapping
(2024-12-09)Fluorescence microscopy facilitates biological research by enabling straightforward visualization of fluorescently labeled proteins in live and fixed samples. Despite significant progress in microscopy techniques, including ... -
PUCK: Primer Utilised CRISPR/Cas Knock-Ins for High Throughput Super-Resolution Microscopy
(2024-11-29)The insertion of tags into proteins via gene editing is a powerful tool for microscopy, enabling the comparative analysis of different proteins. The advent of CRISPR/Cas systems has greatly simplified gene editing and ... -
Dynamics and regulation of the mitochondrial translation machinery
(2024-11-15)Throughout this project, we have successfully established and implemented a ribosome profiling protocol tailored for examination of translating mitochondrial ribosomes, thereby affording a deeper understanding of mitochondrial ... -
Depletion of respiratory chain complexes modulates cristae architecture via MIC10 in human cells
(2024-10-18)The complex structure of the inner mitochondrial membrane plays a key role in mitochondrial function. The inner mitochondrial membrane is divided into the inner boundary membrane and the cristae membrane, which is characterized ... -
Investigation of the Mitochondria-ER axis and its interconnection with the MICOS complex
(2024-09-19)Mitochondria-ER contact sites (MERCS) are essential cellular hubs for the exchange of signaling molecules, lipids and metabolites. A multitude of MERCS-associated proteins have been described in mammalian cells, and rising ... -
Characterization of testosterone and androgen receptor action in human and rhesus macaque heart muscle cells
(2024-03-26)Androgens are sex steroid hormones that influence various processes in reproductive tissues and peripheral organs. Their lipophilic nature enables free diffusion through the plasma membrane. In the cytoplasm, testosterone ... -
Investigation of OPA1 in Mitochondrial Dynamics and Ultrastructure
(2024-03-21)Mitochondria are highly dynamic organelles with a complex ultrastructure. The mitochondrial inner membrane (MIM) is folded into cristae in which oxidative phosphorylation, a process to generate energy for the cell, takes ... -
Automated STED microscopy for cell-biological high-throughput assays
(2022-11-28)In the past decade, super-resolution fluorescence microscopy has revolutionized biological research by enabling the study of biological functions down to the molecular scale. Despite profound technological developments, ... -
Investigation of early assembly of OXPHOS complexes during mitochondrial translation
(2020-12-02)After a long time of coevolution between mitochondria and their eukaryotic host cells, the mitochondrial genome has diverged severely from it’s α- proteobacterial ancestors. Mitochondrial genes have mainly been transferred to ... -
Multicolor 3D MINFLUX nanoscopy for biological imaging
(2020-11-12)The resolution of conventional optical fluorescence far-field microscopes is limited by the diffraction of light. This implies that only features in a distance of about half of the wavelength can be discerned. In the last ... -
Insights into mitochondrial presequence and carrier import pathways
(2020-10-20)The mitochondria of yeast Saccharomyces cerevisiae contains more than 1000 proteins, majority of which are imported from the cytosol. The TIM23 and the TIM22 complexes in the inner mitochondrial membrane are essential ... -
Development, characterization, and application of RNA catalysts for in situ labeling of target RNA molecules
(2020-08-07)RNA molecules play a diverse set of crucial roles in biological systems. Studying various aspects of RNA biology such as RNA transport, localization, degradation, and structural dynamics is therefore of utmost significance. ... -
Analysis of the Interactome and Membrane Insertion of VAPB, a Tail- Anchored Protein at the Inner Nuclear Membrane
(2020-06-26)Tail-anchored (TA) proteins are a group of integral membrane proteins defined by the presence of a single transmembrane domain (TMD) at the C-terminal domain and are involved in functionally diverse cellular processes. ... -
Development and characterization of novel reversibly switchable red fluorescent proteins with opposing switching modes
(2020-03-03)Since the discovery of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria, a multitude of fluorescent proteins have been derived from the original GFP and its homologs by protein engineering. Their ... -
Optimization and application of Trim-Away for studying a liquid-like spindle domain in mammalian oocytes
(2019-10-01)In this thesis, I reported a membraneless structure that permeated a major region of the female meiotic spindle and formed droplet-like protrusions around the spindle poles. Proteins within this previously unknown structure ... -
Molecular investigation of mitochondrial inner membrane morphology
(2019-06-03)The mitochondrial inner membrane (MIM) is highly curved and displays a complex but extremely organised ultrastructure. It is subdivided into two morphologically, topologically, as well as functionally, distinct compartments. ... -
Study of a kinesin adaptor in axonal transport and synapse formation
(2019-02-25)To form a synaptic terminal, various components have to be targeted to specific sites in the axon. Presynaptic proteins are synthesized in the cell soma, packaged in vesicular cargos and transported by the motor complexes ... -
Molecular Characterization of the Mitochondrial Presequence Translocase
(2018-01-31)In this thesis, multiple pore-lining amino acid residues of the mitochondrial presequence translocation channel Tim23 were identified as constituents of the channel’s cation filter by combining single channel electrophysiology ... -
CRISPR-Cas9-mediated protein tagging in human cells for RESOLFT nanoscopy and the analysis of mitochondrial prohibitins
(2016-12-13)Ectopic overexpression of fluorescent fusion proteins for live cell imaging studies often leads to a multitude of artefacts, but protein expression at endogenous levels in mammalian cells was difficult to achieve so far. ... -
Characterization of inner mitochondrial membrane architecture
(2016-08-26)The inner membrane of mitochondria is extensively folded and displays a complex architecture. Cristae junctions are highly curved tubular openings that separate the cristae membrane from the inner boundary membrane. They ...