Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Köster, Sarah Prof. Dr."
Now showing items 1-20 of 27
-
CFM: Confinement Force Microscopy-a dynamic, precise and stable microconfiner for traction force microscopy to study confined cell behaviour
(2024-01-11)Physical properties of the 3D microenvironment and the residing tissue affect cell behavior and function. 3D environment represents major physical obstacles for cell migration, such as spatial confinement, which could ... -
Mechanics and Motility of Epithelial Cells: From Single Cell Behavior to Collective Migration
(2023-01-13)Mechanical behavior of cells plays a crucial role in a plethora of biological processes. Despite its importance, this aspect of life science has only more recently gained increasing interest. Yet, without mechanical force ... -
Automated STED microscopy for cell-biological high-throughput assays
(2022-11-28)In the past decade, super-resolution fluorescence microscopy has revolutionized biological research by enabling the study of biological functions down to the molecular scale. Despite profound technological developments, ... -
Dynamics and self-organization of membrane-bound minimal actin and actomyosin cortices based on ezrin-PtdIns[4,5]P2 linkage
(2022-11-22)The cellular cortex is a thin actin layer attached to the cytoplasmatic plasma membrane leaflet of mammalian cells. Through a complex interaction with hundreds of actin binding proteins (ABPs), controlling e.g. the cortex ... -
Modelling the Dynamics of Actomyosin Networks
(2022-11-04)In this dissertation, a new framework called bead-state model (BSM) for computer simulations of actin networks is presented, and its scientific validity and its usability and flexibility is demonstrated in three ... -
Intermediate filament mechanics across scales
(2022-02-24)The mechanical properties of cells are largely determined by the cytoskeleton. The cytoskeleton is an intricate and complex structure formed by protein filaments, motor proteins, and crosslinkers. The three main types of ... -
Multicolor 3D MINFLUX nanoscopy for biological imaging
(2020-11-12)The resolution of conventional optical fluorescence far-field microscopes is limited by the diffraction of light. This implies that only features in a distance of about half of the wavelength can be discerned. In the last ... -
Collective Mechanical Behavior of Epithelial Cells - The Impact of Micro-Wounding
(2019-06-24)For epithelia, which are lining organs or blood vessels, the mechanical integrity of a whole cell sheet is indispensable. The question arises, how neighboring cells mechanically work together to build a functioning cell ... -
Microfluidic cryofixation for time-correlated live-imaging cryo-fluorescence microscopy and electron microscopy of Caenorhabditis elegans
(2018-11-01)Light and electron microscopy are complementary methods to study biological systems at the cellular and sub-cellular scale. Light microscopy is compatible with live cells, allowing features of interest to be selectively ... -
Self-Organization and Mechanics of Minimal Actin Cortices attached to artificial Bilayers
(2018-10-23)One of the most versatile used protein structure in nature are actin filaments. They form contractile structures, like muscle fibrils, as well as complex network structures like the cellular cortex. The actin cortex is ... -
The Influence of Substrate Elasticity and Shear Rate on Human Blood Platelet Contraction
(2018-09-12)Human blood platelets are small, anucleate cell fragments that are important for the life-saving process of haemostasis, i.e. the process of wound healing. Upon injury, blood platelets adhere to the exposed extra-cellular ... -
Stress-Strain Behavior of Single Vimentin Intermediate Filaments
(2018-08-09)Cells are the basic unit of living organisms and consist of a cytoplasm, which is enclosed by a membrane. As building blocks of life with a plethora of functions, cells have to be equipped with a high degree of mechanical ... -
Active and Passive Microrheology of F-Actin Membrane Composites
(2018-07-19)The complex mechanical properties of a living cell are not only a function of its structural components but also of the organizational super-structure and the dynamic interconnection of filaments, proteins and the plasma ... -
STED Microscopy of FRET Pairs
(2018-05-18)Förster resonance energy transfer (FRET) is a popular tool in life sciences, for example to detect protein-protein interactions and ligand binding, or to construct fluorescent biosensors for metabolites or ions. Obtaining ... -
Fluorescence correlation spectroscopy for studying intermediate filament assembly
(2017-08-10)Intermediate filaments play a central role in the cytoskeleton of eukaryotic cells. Together with microtubules and actin filaments they determine the mechanical properties of cells. Microtubules are also the guiding tracks ... -
Tracking Assembly Kinetics of Intermediate Filaments
(2017-04-13)Vimentin is an intermediate filament (IF) protein in eukaryotic cells and together with other IFs, actin filament and microtubules plays an instrumental role in providing stability and mechanical properties to the cell. ... -
Membrane tension homeostasis of mammalian cells
(2017-04-12)Although a cell is the smallest unit that is viable on its own, its construction and processes are of great complexity. Many functions, which keep a cell alive, are only poorly understood up to now. Plasma membrane tension ... -
STED Microscopy with Scanning Fields Below the Diffraction Limit
(2016-11-17)Since its development, STED microscopy has been used extensively for imaging biological samples. To label structures of interest, fluorescent proteins and organic dyes are used almost exclusively. However, these labels ... -
Structure and dynamics of stress fibers in adult stem cells
(2016-07-19)Since decades, the differentiation potential of adult human mesenchymal stem cells (hMSCs) is investigated. They feature the ability for differentiation into various cell types, like cartilage, fat, nerve, muscle and ... -
Dynamics of Surfactants at Soft Interfaces using Droplet-Based Microfluidics
(2016-01-26)In this work, I studied the impact of surfactants on interfacial phenomena out of equilibrium. I investigated three different effects, (1) the stabilisation of interfaces upon surfactant adsorption (adsorption kinetics), ...