Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Kehlenbach, Ralph Prof. Dr."
Now showing items 1-19 of 19
-
Dynamics and regulation of the mitochondrial translation machinery
(2024-11-15)Throughout this project, we have successfully established and implemented a ribosome profiling protocol tailored for examination of translating mitochondrial ribosomes, thereby affording a deeper understanding of mitochondrial ... -
Investigation of the Mitochondria-ER axis and its interconnection with the MICOS complex
(2024-09-19)Mitochondria-ER contact sites (MERCS) are essential cellular hubs for the exchange of signaling molecules, lipids and metabolites. A multitude of MERCS-associated proteins have been described in mammalian cells, and rising ... -
Characterization of the stepwise maturation of the telomerase RNA TLC1 and assembly of the telomerase in Saccharomyces cerevisiae
(2021-11-04)Telomere length homeostasis is a prerequisite for cell viability. It is challenged through successive shortening, which is due to the “end-replication-problem”. This limits the cellular life span in multicellular organisms ... -
Investigation of Mitochondrial Ribosome Regulators at the Inner Membrane
(2021-09-24)Mitochondrial biogenesis relies on the proper assembly of proteins from dual genetic origin. Nuclear-encoded proteins are imported into the organelle and incorporated into multiprotein complexes together with proteins ... -
Dissecting the molecular mechanism and spatiotemporal dynamics controlling senescence entry
(2021-01-21)Senescence represents a programmed cell cycle arrest against aberrant proliferation and tumour development due to accumulated genotoxic or metabolic stress. The tumour-suppressive properties of senescence attracted the ... -
Insights into the biogenesis of the human mitochondrial ribosomal large subunit – Characterisation of mL44 and mL45
(2020-11-16)Due to the presence of high-resolution cryo-EM structures of the human mitoribosome, the understanding about the function and assembly of the intriguing mitochondrial ribonucleoprotein complex increased tremendously during ... -
Aspergillus fumigatus F-box protein Fbx15 functions are dependent on its nuclear localisation signals and are partially conserved between A. fumigatus and A. nidulans
(2020-09-08)Aspergillus fumigatus is a globally distributed opportunistic filamentous fungal pathogen mainly found in compost and represents the main cause of pulmonary aspergillosis in immunocompromised individuals. Fungal development ... -
Structure Based Search for Novel Nuclear Export Inhibiting Anti-Tumor Drugs
(2020-07-28)The intracellular distribution of proteins and ribonucleoproteins is a fundamental process in all eukaryotes that is critically vital for the normal functionality of the eukaryotic cell. In the last decades, it has been ... -
The Aspergillus fumigatus Vap-Vip methyltransferase pathway modulates stress response, secondary metabolism and azole resistance
(2019-07-11)The VapA-VapB-VipC trimeric complex is part of an epigenetic developmental control pathway of A. nidulans. VeA-interacting protein (VipC) and VipC-associated protein B (VapB) are methyltransferases, and VapA is a zinc-finger ... -
The role of mammalian TRC40 in membrane-protein targeting and chaperoning
(2019-05-24)Tail-anchored (TA) proteins are distinguished from other membrane proteins due to their particular topology. The best-characterized pathway for the targeting of TA-proteins is the GET pathway in yeast or the TRC pathway ... -
Maturation and nucleo-cytoplasmic shuttling of snRNAs in Saccharomyces cerevisiae
(2019-01-24)While nucleo-cytoplasmic shuttling of snRNAs is a well known process in higher eukaryotes, it is still unknown whether snRNAs shuttle in Saccharomyces cerevisiae. Furthermore, it is unclear why snRNA shuttling evolved in ... -
Molecular Characterization of the Mitochondrial Presequence Translocase
(2018-01-31)In this thesis, multiple pore-lining amino acid residues of the mitochondrial presequence translocation channel Tim23 were identified as constituents of the channel’s cation filter by combining single channel electrophysiology ... -
Role of WRB protein in cardiac function
(2017-08-24)The mammalian TRC40 system is a conserved pathway responsible for the post-translational targeting of tail-anchored proteins, which are characterized by a single transmembrane domain at the C-terminus and an N-terminus ... -
Characterization of mRNA export and nuclear quality control under heat stress in the yeast Saccharomyces cerevisiae
(2017-05-17)Elevated temperatures and other changes of the environment a cell lives in, can have tremendous impact on cellular processes and integrity. To counteract these threats, cells have established various mechanisms to avoid ... -
Transport of Tail-anchored Proteins to the Inner Nuclear Membrane
(2017-04-13)In contrast to the well-studied process of nucleocytoplasmic transport of soluble macromolecules, little is known about the targeting of integral membrane proteins to the inner nuclear membrane (INM), therefore the targeting ... -
Characterization of inner mitochondrial membrane architecture
(2016-08-26)The inner membrane of mitochondria is extensively folded and displays a complex architecture. Cristae junctions are highly curved tubular openings that separate the cristae membrane from the inner boundary membrane. They ... -
Atg26 is involved in selective autophagy of the major coat protein Gag of the S. cerevisiae virus L-A
(2016-01-22)Macroautophagy is a conserved catabolic pathway for the removal and recycling of cytosolic components, damaged or surplus organelles, protein aggregates or intracellular pathogens to maintain cellular homeostasis. It is ... -
Paramagnetic tools for the structural analysis of high molecular weight proteins
(2015-12-10)Paramagnetic effects provide important structural and dynamic information of biomolecules. However, the attachment of lanthanides through small chelating organic molecules to proteins (the most common way to obtain ... -
Role of Histone Metabolism and Chromatin Structure in DNA Repair
(2014-06-11)During the cell cycle progression, synthesis of new histones is important to pack newly synthesized DNA and to maintain proper chromatin structure. Unlike normal mRNAs, mRNAs from replication-dependent histone genes that ...