Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Lührmann, Reinhard Prof. Dr."
Now showing items 1-20 of 20
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Structural Insights into Early Spliceosome Assembly by cryo-EM
(2021-12-23)In eukaryotes, splicing is a process during which the non-coding introns of the pre-messenger RNA (pre-mRNA) are removed, and the protein-coding exons are ligated. The introns are defined by conserved 5' splice site (5'SS), ... -
Bioinformatics analysis of multi-omics data elucidates U2 snRNP function in transcription
(2021-08-16)Transcription by RNA polymerase II (Pol II) is an important step in cell function and regulation. Pol II transcription has been shown to be coupled to pre-mRNA splicing, but the underlying mechanisms remain poorly ... -
Investigation of the effects of the splicing inhibitor Pladienolide B and cancer-related mutations in the SF3b1 protein on pre-mRNA splicing in vitro
(2020-06-11)Pre-mRNA splicing is catalyzed by the spliceosome. A highly dynamic, macromolecular RNP complex is formed out of the U1, U2, U4, U5 and U6 snRNPs and multiple snRNP proteins. During the early stages of spliceosome assembly, ... -
Biophysical and Crystallographic Characterization of Spliceosomal DExD/H-box ATPases
(2020-04-23)DEAH-box ATPases play a vital role in the activation, catalytic and disassembly steps of the spliceosome. They are key for the orchestration of conformational and compositional rearrangements during the splicing cycle. It ... -
Gbp2 and Hrb1 continue their mRNA quality control in the cytoplasm and take part in Nonsense Mediated Decay
(2019-09-02)The eukaryotic mRNA life cycle is orchestrated by many pathways. Every aspect of this life cycle is associated with quality control factors. This tight coupling of mRNA transcription, processing, export and translation ... -
Smu1 and RED play an important role for the activation of human spliceosomes
(2019-07-08)In eukaryotes, pre-mRNA splicing is catalysed by the spliceosome, a highly complex and dynamic molecular machine, which assembles stepwise by the sequential recruitment of five small nuclear ribonucleoproteins (snRNPs) and ... -
Molecular characterization and functional analysis of a novel long noncoding RNA in the mouse
(2019-05-24)Majority of mammalian transcriptomes encode long noncoding RNAs (lncRNAs) that are not translated into proteins. LncRNAs are mRNA-like transcripts that are longer than 200 nucleotides, transcribed by RNA-Pol-II and undergo ... -
High-resolution structure determination of human spliceosome complexes by cryo-EM
(2019-02-14)The eukaryotic splicing of precursors to mRNA is facilitated by a highly dynamic, multi-megadalton macromolecular machine termed the spliceosome. The underlying chemical reaction features the excision of an intron, which ... -
Functional characterization of C/D snoRNA-derived microRNAs
(2018-06-26)miRNAs are essential regulators of cell fate and involved in several human diseases, although their biogenesis is a well-accepted paradigm (microprocessor, Exp5, Dicer, and Argonaute). New evidence revealed different ... -
Structural and functional investigation of cargo recognition by exportins
(2016-11-02)Nucleocytoplasmic exchange is essential for eukaryotic cells. It proceeds through nuclear pore complexes and is largely mediated by Importin β-like nuclear transport receptors (Impβ-like NTRs). According to the direction ... -
Requirements for pre-catalytic B complex formation during exon- and intron-defined spliceosome assembly
(2015-10-07)In eukaryotes, most protein-coding genes are interrupted by non-coding sequences known as introns. The splicing of intronic sequences from a pre-mRNA is catalyzed by the spliceosome, an elaborate molecular machine formed ... -
Crystallization and biophysical characterization of spliceosomal protein complexes
(2015-02-20)mRNA splicing is a highly dynamic process by which non-coding sequences are removed from a pre-mature mRNA. It is catalyzed by the spliceosome, a huge and highly dynamic ribonucleoprotein complex consisting of up to five ... -
Characterization of the Dynein-Dynactin Interaction
(2015-02-13)In eukaryotic cells many biological processes like cell division, the distribution of vesicles and organelles require the dynamic rearrangement of molecules and cellular components. These rearrangements are controlled by ... -
Biochemical characterization of the Nup62⋅58⋅54 nucleoporin complex and mutational analysis of the exportin CRM1
(2014-10-15)Nuclear pore complexes (NPCs) are giant molecular machines embedded in the double membrane that surrounds the eukaryotic nucleus. Deciphering the molecular structure of the NPCs is critical to our understanding of both ... -
Functional characterization and application of 2',5'- branched RNA forming deoxyribozymes using lanthanides as cofactors
(2013-12-13)Deoxyribozymes or DNA catalysts are synthetic single stranded DNA molecules capable of catalysis for a wide range or reactions. Deoxyribozymes are discovered by in vitro selection and are mostly dependent on bivalent metal ... -
Structural insights into Arginine-Serine rich proteins and N-H spin-spin coupling constants
(2013-11-27)This thesis is constituted of two parts. In the first part of this thesis, I studied the structural phenomena of SR protein phosphorylation. The serine/arginine-rich (SR) protein family plays multiple functions in the whole ... -
Insights into the activation of the spliceosomal helicase Prp43
(2013-11-20)The excision of non-coding sequences that are present in the pre-mRNA is a hallmark of eukaryotic cells. This process is termed as splicing and is catalysed by the spliceosome, a single-turnover enzyme which consists of ... -
Single molecule fluorescence spectroscopy of the structure and dynamics of the spliceosome
(2013-11-05)Binding reactions can be ideally investigated by Dual-Color Fluorescence Cross-Correlation Spectroscopy (FCCS). Here, this method is used to study the recruitment and dissociation of proteins to and from the spliceosome. ... -
Methods to improve the sample quality of macromolecular complexes for structure determination by 3D Electron Cryo-Microscopy
(2012-05-15)Macromolecular assemblies often undergo substantial structural rearrangements. Not only do functional states differ from each other, a single functional state also exhibits inherent conformational heterogeneity. A typical ... -
Biochemical and functional differences of chromatin assembled replication-coupled or independent in Xenopus laevis egg extracts
(2012-04-12)Two main chromatin assembly pathways ensure the proper transmission of chromatin organization and chromatin-based information throughout the cell cycle. A replication-dependent (RD) pathway that couples chromatin assembly to ...