Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Rizzoli, Silvio O. Prof. Dr."
Now showing items 1-20 of 25
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Nanoscale organization of CaV1.3 L-type Ca2+ channel clusters in electrically excitable cells
(2024-06-18)A key player of excitable cells in the heart and brain is the L-type calcium channel CaV1.3, which translates electrical signals into a cellular response. In the heart, CaV1.3 holds a central role in pacemaking, signal ... -
Quantitative Nanoscopy of Synaptic Sites
(2023-08-23)Synapses are the basic information processing unit of the brain. Neuronal activity and memory formation thereby require a vastly dynamic and rapidly changing synaptic environment and composition. In order to function, these ... -
Ultrastructural study of synaptic vesicle dynamics in murine inner hair cell ribbon synapses under sustained stimulation
(2023-07-11)Transmission of auditory information relies in the correct functioning of inner hair cell (IHC) rib-bon synapses, which mediate synaptic vesicle release in an indefatigable manner. The name-giving synaptic ribbon and the ... -
Characterizing intracellular lipid signaling in dilated cardiomyopathy using human iPSC-derived cardiac cells
(2023-04-18)Sarcomere protein misalignment is a primary consequence of inherited mutations causing dilated cardiomyopathy (DCM) and results in severe molecular patho-phenotypes in cardiomyocytes from patients. In this study, human ... -
Mechanics and Motility of Epithelial Cells: From Single Cell Behavior to Collective Migration
(2023-01-13)Mechanical behavior of cells plays a crucial role in a plethora of biological processes. Despite its importance, this aspect of life science has only more recently gained increasing interest. Yet, without mechanical force ... -
Dynamics and self-organization of membrane-bound minimal actin and actomyosin cortices based on ezrin-PtdIns[4,5]P2 linkage
(2022-11-22)The cellular cortex is a thin actin layer attached to the cytoplasmatic plasma membrane leaflet of mammalian cells. Through a complex interaction with hundreds of actin binding proteins (ABPs), controlling e.g. the cortex ... -
Modifications in AP2 and clathrin-mediated endocytosis as a mechanism of synaptic plasticity
(2022-07-01)AP2 CME fulfills essential functions in embryonic development and cell survival. The AP2 complex and clathrin form AP2 CCV together with sixty other coat proteins. Due to this complex protein network, the regulation of CCV ... -
Regulation of vesicular trafficking in presence of mutations in contractile proteins
(2022-06-20)The molecular and cellular mechanisms underlying disease phenotypes in dilated cardiomyopathy (DCM) are diverse and to date incompletely understood. Inherited mutations in genes encoding structural components of the sarcomere ... -
Mitochondrial adaptation at the neuronal presynapse
(2021-11-22)Synaptic transmission poses a major energy consuming process in the brain, but how neurons maintain a constant energy supply during extended synaptic activity and how presynaptic mitochondria contribute to the energy ... -
Novel design and data analysis concepts for MINFLUX nanoscopy
(2021-11-22)MINFLUX is a novel concept for super-resolution fluorescence microscopy that achieves unprecedented image resolution despite the limited photon budget of fluorescent molecules. The goal of this thesis is to improve MINFLUX ... -
Signalling from Endosomes in Neurodevelopment and Neurodegenerative Disorders
(2021-11-15)The nervous system is developed under the regulation of growth and guidance cues. This process is highly dynamic and tightly regulated by the composition, concentration and timely presence of different signalling molecules. ... -
Development of a versatile microfluidic platform to study the ionic mechanisms of vesicular neurotransmitter transporters
(2021-11-04)This thesis described the development of a novel, versatile microfluidic platform for studying the ionic mechanisms of vesicular neurotransmitter transporters in rodent synaptic vesicles (SVs). The level of filling of an ... -
Targeting microglial CSF1R in health and disease
(2021-09-03)The main effector functions of microglia are immune, synaptic network refinement, brain insult reaction, and neurogenesis regulation. This cumulative doctoral thesis focuses on the relevance of the colony-stimulating factor ... -
Design strategies for carbon nanotube-based biosensors
(2020-07-09)Global health crises such as the COVID-19 pandemic clearly show the need for novel and better diagnostic and therapeutic tools. Additionally, understanding underlying molecular processes is crucial. However, current methods ... -
A quantitative analysis of an average cultured neuron
(2020-04-02)Neurons are the functional unit of the brain. They have been extensively studied, from a molecular perspective up to brain wide levels. Nonetheless, we are still missing a quantitative description of the neuronal components. ... -
Brain-wide connectivity of molecularly defined GABAergic neurons in mouse barrel cortex visualized with optimized rabies virus tracing
(2020-03-16)Cortical GABAergic neurons are indispensable in controlling the activity of cortical networks. Parvalbu- min (PV), somatostatin (SST) and vasoactive intestinal polypeptide (VIP) expressing neurons are three main classes ... -
The molecular anatomy of synaptic vesicle recycling at the hair cell ribbon synapse
(2019-11-19)The inner hair cell (IHC) ribbon synapse of the auditory system is a highly specialized synapse, adapted to very fast transmission rates and maintenance of synaptic vesicle release over long periods of time without showing ... -
Structure and Mechanics of Neuronal Model Systems
(2019-11-15)Neurons of advanced animals need fast and efficient mechanisms to enable the functionality of a complex nervous system. In the life cycle of synaptic vesicles this is reflected in the endocytosis which generates new vesicles, ... -
Investigating post-translational modifications and novel interaction partners of otoferlin
(2019-10-30)Otoferlin is a large multi-C2-domain protein essential for hearing and fast Ca2+-triggered transmitter release from auditory IHCs. Mutations in the OTOF gene are linked to a form of autosomal recessive non-syndromic hearing ... -
A quantitative analysis of the molecular organization of dendritic spines from hippocampal neurons
(2019-08-20)Dendritic spines are the major excitatory postsynaptic compartment in the brain. Despite its essential function in synaptic transmission and plasticity, we are still lacking a comprehensive and quantitative understanding ...