Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Steinem, Claudia Prof. Dr."
Now showing items 1-20 of 34
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Fluorescence Correlates of Single Vesicles to Analyze the Behavior of Membrane-manipulating Proteins
(2025-01-24)Soluble NSF-attachment protein receptors (SNAREs) are integral to eukaryotic membrane fusion, providing the necessary energy to surmount the repulsive forces between two opposing membranes. This is achieved through the ... -
Investigation of the fusion machinery reconstituted into novel pore-spanning membranes
(2024-09-06)The release of neurotransmitter into the synaptic cleft is achieved by fusion of synaptic vesicles with the presynaptic membrane. This essential process in the neuronal signal transmission is driven by the soluble ... -
Molecular characterization of protein translocation pores
(2024-02-27)Within the intricate realm of intracellular protein transport, individual organelles are reliant upon precise and dedicated protein machineries, commonly referred to as translocon, to facilitate the translocation of proteins ... -
Mechanistic Investigation of the Mode of Action of the Antimicrobial Peptide Lugdunin in Lipid Membranes
(2024-01-26)Dissemination of infections originating from antibiotic-resistant bacteria constitutes an escalating threat to global healthcare systems further compounded by the stagnant development of novel antibiotics. Therefore, the ... -
Giant plasma membrane vesicle-derived in vitro systems to study the structure and dynamics of CaV1.3 channel clusters
(2023-11-06)The voltage-gated calcium channel 1.3 (CaV1.3) has plentiful tasks in the human body. One of them is the excitation-contraction coupling within the heart, where it is known to form CaV1.3 channel cluster. However, their ... -
Molecular Dynamics Studies of Permeation and Gating Mechanisms in Potassium Channels
(2023-04-19)Potassium (K+) channels contribute to diverse physiological activities, representing an indispensable part of living organisms. A thorough understanding of different aspects of permeation mechanisms and gating in K+ channels ... -
Dynamics and self-organization of membrane-bound minimal actin and actomyosin cortices based on ezrin-PtdIns[4,5]P2 linkage
(2022-11-22)The cellular cortex is a thin actin layer attached to the cytoplasmatic plasma membrane leaflet of mammalian cells. Through a complex interaction with hundreds of actin binding proteins (ABPs), controlling e.g. the cortex ... -
Droplet-based giant unilamellar vesicles as a tool for pore-spanning membrane preparation and transmembrane protein reconstitution
(2022-11-22)Model membranes represent a central tool to decipher complex processes of living cells. Giant unilamellar vesicles (GUVs) are of particular interest as they are cell-like in size, observable with optical microscopy and ... -
Targeting ion sensors to synaptic vesicles using a Nanobody against luminal Synaptotagmin 1
(2022-02-24)The synapse is a specialized nanostructure that connects two neurons where the ionic composition in each of its nano compartments is the driving process for synaptic transmission. Synaptic transmission relies on the ... -
Quantifying topological transformation in lipid self-assemblies
(2021-06-03)Surfactant assemblies are characterized by soft intermolecular interactions and can be deformed or topologically altered by thermal or mechanical stress of the magnitude of thermal fluctuations. In biology, the protein ... -
SNARE-mediated membrane fusion on pore-spanning membranes – several fusion pathways analyzed by single-vesicle content release
(2021-01-08)The fusion of neurotransmitter filled vesicles with the presynaptic membrane is the key step in the neuronal signaling cascade and is mediated by soluble N-ethylmaleimide-sensitive factor attachment receptor proteins ... -
On the molecular basis of α-synuclein aggregation on phospholipid membranes in the presence and absence of anle138b
(2020-07-22)A physiologic hallmark of synucleinopathies such as Parkinson’s Disease (PD) or Multiple System Atrophy (MSA) are deposits in patients’ brains, which consist of the α-synuclein. Growing evidence suggests, that oligomeric ... -
Design strategies for carbon nanotube-based biosensors
(2020-07-09)Global health crises such as the COVID-19 pandemic clearly show the need for novel and better diagnostic and therapeutic tools. Additionally, understanding underlying molecular processes is crucial. However, current methods ... -
Reconstitution of retrotranslocation by the Hrd1 ubiquitin ligase with purified components
(2020-03-11)In eukaryotic organisms, a large fraction of newly-synthesized proteins are destined for the endomembrane system or for secretion. The entry gate for these proteins is the endoplasmic reticulum (ER). Proteins are imported ... -
Quantification of forces involved in carbohydrate induced attachment of carcinoma cells
(2020-02-26)Vital biological processes such as the adhesion of cells to their surroundings rely on short-term recognition processes, which are defined by a rapid assembly and disassembly of adhesion molecules. These processes were ... -
Lipid dependent interactions of biomimetic membrane models with the ENTH domain of epsin
(2020-02-05)Membrane remodeling processes during endocytosis are highly regulated by the protein-protein and protein-membrane interactions. In clathrin-mediated endocyto-sis (CME) binding of the protein epsin to its receptor lipid ... -
Self-Organization and Mechanics of Minimal Actin Cortices attached to artificial Bilayers
(2018-10-23)One of the most versatile used protein structure in nature are actin filaments. They form contractile structures, like muscle fibrils, as well as complex network structures like the cellular cortex. The actin cortex is ... -
The Influence of Substrate Elasticity and Shear Rate on Human Blood Platelet Contraction
(2018-09-12)Human blood platelets are small, anucleate cell fragments that are important for the life-saving process of haemostasis, i.e. the process of wound healing. Upon injury, blood platelets adhere to the exposed extra-cellular ... -
Pore-spanning membranes – a versatile tool to analyze SNARE-mediated single vesicle fusion
(2018-06-27)Signal transmission in neurons and hormone secretion in neuroendocrine cells such as chromaffin cells are fundamental biological processes including SNARE- (soluble N-ethylmaleimide-sensitive-factor attachment receptor) ... -
Mechanoelectrical Coupling and Reorganisation of Cardiomyocytes and Fibroblasts under Shear Stress
(2018-05-29)In this work, I studied the influence of changes such as the abundance of myofibroblasts in fibrotic conditions and the increased shear stress on cellular mechanical properties, morphology, contractility and connectivity. By ...