Tumor invasion through 3D engineered basement membrane

by Zhiming Zhao
Doctoral thesis
Date of Examination:2022-04-28
Date of issue:2022-04-14
Advisor:Prof. Dr Arndt Schilling
Referee:Prof. Dr Arndt Schilling
Referee:Prof. Dr. Fred Wouters
Persistent Address: http://dx.doi.org/10.53846/goediss-9180

 

 

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Abstract

English

Tumor metastasis is the most important reason of cancer-related death. The progress of tumor metastasis includes intravasation, survival in the circulation, extravasation and col- onization. In the process of metastasis intravasation, the basement membrane is the first important barrier. Therefore, engineering a basement membrane in 3D structure might provide a novel 3D model of tumor metastasis, leading to a better understanding of tumor intravasation. In this thesis, a novel artificial engineered basement membrane (EBM) was fabricated by gelation of Matrigel matrix and PEGDA 20,000 under UV exposure. The function of this EBM was investigated by MDA-MB-231 and MCF-7 cell invasion in xCELLigence. MDA-MB-231 cells invaded through the membrane successfully but MCF-7 cells failed, showing that the EBM can differentiate between metastatic and non-metastatic tumor cells. The invasion process was significantly influenced by shikonin and irradiation, showing that the EBM allows to study tumor related treatments. In summary, this thesis established a novel 3D in vitro tumor metastasis model which may serve as a platform for drug screening and study of tumor metastasis.
Keywords: tumor invasion; basement membrane; irradiation; 3d printing
Schlagwörter: tumor invasion; basement membrane; irradiation; 3d printing
 
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